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首页> 外文期刊>Materials science & engineering >Poly(3-hydroxybutyrate)/caffeic acid electrospun fibrous materials coated with polyelectrolyte complex and their antibacterial activity and in vitro antitumor effect against HeLa cells
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Poly(3-hydroxybutyrate)/caffeic acid electrospun fibrous materials coated with polyelectrolyte complex and their antibacterial activity and in vitro antitumor effect against HeLa cells

机译：涂有聚电解质复合物的聚3-羟基丁酸酯/咖啡酸电纺纤维材料及其对HeLa细胞的抗菌活性和体外抗肿瘤作用

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摘要

The purpose of this work was to investigate the possibility for the preparation of new poly(3-hydroxybutyrate) (PHB)/poly(ethylene glycol) (PEG)-based fibrous materials containing natural phenolic compound caffeic acid (CA) of diverse architectures, as well as to study the impact of the fiber composition on the in vitro CA release profile and on the biological properties of the fibrous materials. The application of the one-pot electrospinning enabled the fabrication of nanofibrous materials from PHB and PEG loaded with the CA. Materials with targeted design were obtained by coating with polyelectrolyte complex of alginate (Alg) and N,N,N-trimethylchitosan (TMCh). Three different processing paths were used to obtain coated mats: (ⅰ) with CA incorporated in the PHB/PEG core; (ⅱ) with CA embedded in the Alg layer; and (ⅲ) with CA included in the TMCh layer. The in vitro release of CA was modulated by controlling the composition and the architecture of the nanofibrous mats. The performed microbiological screening and MTT cell viability studies revealed that in contrast to the bare mats, the CA-containing nanofibrous materials were effective in suppressing the growth of the Gram-positive bacteria Staphylococcus aureus and the Gram-negative bacteria Escherichia coli and displayed good cytotoxicity against human cervical HeLa tumor cells. In addition, the proliferation of murine spleen lymphocytes and peritoneal macrophages was increased by the prepared CA-containing nanofibrous materials. The obtained materials are promising for antibacterial wound dressing applications as well as for application in local treatment of cervical tumors.
机译：这项工作的目的是研究制备包含多种结构的天然酚类化合物咖啡酸（CA）的新型基于聚（3-羟基丁酸）（PHB）/聚（乙二醇）（PEG）的纤维材料的可能性，以及研究纤维成分对体外CA释放曲线和纤维材料生物学特性的影响。一锅静电纺丝技术的应用使PHB和装有CA的PEG能够制备纳米纤维材料。通过用藻酸盐（Alg）和N，N，N-三甲基壳聚糖（TMCh）的聚电解质复合物涂覆获得具有目标设计的材料。使用三种不同的加工路径来获得涂层毡：（1）在PHB / PEG芯材中掺入了CA。 （ⅱ）CA嵌入在Alg层中； （ⅲ）包含TMCh层中的CA。通过控制纳米纤维垫的组成和结构来调节CA的体外释放。进行的微生物筛选和MTT细胞活力研究表明，与裸露的垫子相比，含CA的纳米纤维材料可有效抑制革兰氏阳性菌金黄色葡萄球菌和革兰氏阴性菌大肠杆菌的生长，并显示出良好的细胞毒性对抗人类宫颈癌HeLa肿瘤细胞。此外，所制备的含CA的纳米纤维材料增加了小鼠脾脏淋巴细胞和腹膜巨噬细胞的增殖。所获得的材料有望用于抗菌伤口敷料应用以及局部治疗宫颈肿瘤。

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  • 来源
    《Materials science & engineering》 |2016年第8期|379-392|共14页
  • 作者

    Milena G. Ignatova; Nevena E. Manolova; Iliya B. Rashkov; Nadya D. Markova; Reneta A. Toshkova; Ani K. Georgieva; Elena B. Nikolova;

  • 作者单位

    Laboratory of Bioactive Polymers, Institute of Polymers, Bulgarian Academy of Sciences, Acad. G. Bonchev St, Bl. 103A, BG-1113 Sofia, Bulgaria;

    Laboratory of Bioactive Polymers, Institute of Polymers, Bulgarian Academy of Sciences, Acad. G. Bonchev St, Bl. 103A, BG-1113 Sofia, Bulgaria;

    Laboratory of Bioactive Polymers, Institute of Polymers, Bulgarian Academy of Sciences, Acad. G. Bonchev St, Bl. 103A, BG-1113 Sofia, Bulgaria;

    Institute of Microbiology, Bulgarian Academy of Sciences, Acad. G. Bonchev Bl. 26, BG-1113 Sofia, Bulgaria;

    Institute of Experimental Morphology, Pathology and Anthropology with Museum, Bulgarian Academy of Sciences, Acad. G. Bonchev St. bl. 25, BG-1113 Sofia, Bulgaria;

    Institute of Experimental Morphology, Pathology and Anthropology with Museum, Bulgarian Academy of Sciences, Acad. G. Bonchev St. bl. 25, BG-1113 Sofia, Bulgaria;

    Institute of Experimental Morphology, Pathology and Anthropology with Museum, Bulgarian Academy of Sciences, Acad. G. Bonchev St. bl. 25, BG-1113 Sofia, Bulgaria;

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  • 正文语种 eng
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  • 关键词

    Electrospinning; Caffeic acid; Poly(3-hydroxybutyrate); Quaternized chitosan; Antibacterial activity; Antitumor activity;

    机译：电纺;咖啡酸;聚（3-羟基丁酸酯）;季铵化壳聚糖;抗菌活性;抗肿瘤活性;

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