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首页> 外文期刊>Materials science & engineering >Gelatin-PMVE/MA composite scaffold promotes expansion of embryonic stem cells
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Gelatin-PMVE/MA composite scaffold promotes expansion of embryonic stem cells

机译：明胶-PMVE / MA复合支架促进胚胎干细胞扩增

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We introduce a new composite scaffold of gelatin and polymethyl vinyl ether-alt-maleic anhydride (PMVE/MA) for expansion of embryonic stem cells (ESCs) in an in vitro environment. To optimize the scaffold, we prepared a gelatin scaffold (G) and three composite scaffolds namely GP-1, GP-2, and GP-3 with varying PMVE/MA concentrations (0.2-1%) and characterized them by scanning electron microscopy (SEM), swelling study, compression testing and FTIR. SEM micrographs revealed interconnected porous structure in all the scaffolds. The permissible hemolysis ratio and activation of platelets by scaffolds confirmed the hemocompatibility of scaffolds. Initial biocompatibility assessment of scaffolds was conducted using hepatocarcinoma (Hep G2) cells and adhesion, proliferation and infiltration of Hep G2 cells in depth of scaffolds were observed, proving the scaffold's biocompatibility. Further Oct4B2 mouse embryonic stem cells (mESCs), which harbor a green fluorescence protein transgene under regulatory control of the Oct4 promotor, were examined for expansion on scaffolds with MTT assay. The GP-2 scaffold demonstrated the best cell proliferation and was further explored for ESC adherence and infiltration in depth (SEM and confocal), and pluripotent state of mESCs was assessed with the expression of Oct4-GFP and stage-specific embryonic antigen-1 (SSEA-1). This study reports the first demonstration of biocompatibility of gelatin-PMVE/MA composite scaffold and presents this scaffold as a promising candidate for embryonic stem cell based tissue engineering.
机译：我们介绍了明胶和聚甲基乙烯基醚-马来酸酐（PMVE / MA）的新型复合支架，用于在体外环境中扩增胚胎干细胞（ESC）。为了优化支架，我们制备了明胶支架（G）和三种复合支架，分别是PMVE / MA浓度（0.2-1％）变化的GP-1，GP-2和GP-3，并通过扫描电子显微镜对其进行了表征（ SEM），溶胀研究，压缩测试和FTIR。 SEM显微照片揭示了所有支架中相互连接的多孔结构。支架的允许溶血率和血小板活化证实了支架的血液相容性。使用肝癌（Hep G2）细胞进行支架的初始生物相容性评估，并观察到支架深处Hep G2细胞的粘附，增殖和浸润，证明了支架的生物相容性。使用MTT分析检测了在Oct4启动子的调控控制下具有绿色荧光蛋白转基因的其他Oct4B2小鼠胚胎干细胞（mESC）在支架上的扩增。 GP-2支架表现出最佳的细胞增殖能力，并进一步探讨了ESC的粘附和深度浸润（SEM和共聚焦），并通过Oct4-GFP和阶段特异性胚胎抗原1（ SSEA-1）。这项研究报告明胶-PMVE / MA复合支架的生物相容性的第一个证明，并提出此支架作为基于胚胎干细胞的组织工程的有希望的候选者。

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