Combinatorial effect of Si~(4+), Ca~(2+), and Mg~(2+) released from bioactive glasses on osteoblast osteocalcin expression and biomineralization

Neda Saffarian Tousi; Megen F. Velten; Timothy J. Bishop; Kelly K. Leong; Nicole S. Barkhordar; Grayson W. Marshall; Peter M. Loomer; Pranesh B. Aswath; Venu G. Varanasi

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Combinatorial effect of Si~(4+), Ca~(2+), and Mg~(2+) released from bioactive glasses on osteoblast osteocalcin expression and biomineralization

机译：生物活性玻璃释放的Si〜（4 +），Ca〜（2+）和Mg〜（2+）对成骨细胞骨钙素表达和生物矿化的组合作用

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Osteocalcin (OCN) expression is an essential osteogenic marker of successful bone regeneration therapies. This study hypothesizes that Si~(4+) and Ca~(2+) combinatorial released by bioactive glass enhance osteoblast biomineralization through up-regulation of OCN expression; and Mg~(2+) release delays such enhancement Oste-oblasts (MC3T3-E1) were treated with ionic products of bioactive glass dissolution (6P53-b experimental bioactive glass and 45S5 commercial Bioglass™). Results showed that gene expressions, including OCN and its up-stream transcription factors (Runx2, ATF4, MSX1, SP7/OSX), growth factors and signaling proteins (BMP2, BMP6, SMAD3)were enhanced in both 45S5 and 6P53-b glass conditioned mediums (GCMs). This up-regulation led to enhanced mineral formation by 45S5 glass conditioned mediums ([GCM], Si~(4+) + Ca~(2+)) after 20 days,and by 45S5 GCM and 6P53-bGCM(Si~(4+) + Ca~(2+) + Mg~(2+)) after 30 days. In examining the extracellular matrix generated by cells when exposed to each GCM, it was found that 45S5 GCM had slightly elevated levels of mineral content within ECM as compared to 6P53-b GCM after 30 days while control treatments exhibited no mineral content. The formation of well-defined mineralized nodules (distinct PO~(3-)_4 [960 cm~(-1)] and CO~(2-)_3 (1072 cm~(-1)] peaks from Raman Spectra) was observed for each GCM as the soluble glass content increased. In examining the individual and combined ion effects between Si~(4+) + Ca~(2+) + Mg~(2+), it was found Mg~(2+) down-regulates OCN expression. Thus, ions released from both 45S5 and 6P53-b bioactive glasses up-regulate OCN expression and biomineralization while 6P53-b GCM Mg~(2+) release down-regulated OCN expression and delayed osteoblast biomineralization. These results indicate that Si~(4+), Ca~(2+), and Mg~(2+) combinato-rially regulate osteoblast OCN expression and biomineralization.

机译：骨钙素（OCN）表达是成功的骨再生疗法的重要成骨标记。这项研究假设生物活性玻璃释放的Si〜（4+）和Ca〜（2+）组合物通过上调OCN表达来增强成骨细胞的生物矿化作用。和Mg〜（2+）释放延迟，以生物活性玻璃溶解的离子产物（6P53-b实验生物活性玻璃和45S5商业Bioglass™）处理这种增强的成骨细胞（MC3T3-E1）。结果表明，在45S5和6P53-b玻璃条件下，包括OCN及其上游转录因子（Runx2，ATF4，MSX1，SP7 / OSX），生长因子和信号蛋白（BMP2，BMP6，SMAD3）的基因表达均得到增强。媒介（GCM）。这种上调导致20天后通过45S5玻璃条件培养基（[GCM]，Si〜（4+）+ Ca〜（2 +）），45S5 GCM和6P53-bGCM（Si〜（4 30天后+）+ Ca〜（2+）+ Mg〜（2+））。在检查细胞暴露于每个GCM时产生的细胞外基质时，发现30天后45S5 GCM与6P53-b GCM相比，ECM中的矿物质含量略有升高，而对照处理则没有矿物质含量。观察到形成明确的矿化结节（拉曼光谱的PO〜（3-）_ 4 [960 cm〜（-1）]和CO〜（2-）_ 3（1072 cm〜（-1）]峰）。随着可溶性玻璃含量的增加，每一个GCM的Mg〜（2+）下降了。调节OCN的表达，因此，从45S5和6P53-b生物活性玻璃中释放的离子上调OCN的表达和生物矿化，而6P53-b GCM Mg〜（2+）释放下调的OCN表达并延迟成骨生物矿化。 Si〜（4 +），Ca〜（2+）和Mg〜（2+）共同调节成骨细胞OCN的表达和生物矿化作用。

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来源
《Materials science & engineering》 |2013年第5期|2757-2765|共9页
作者
Neda Saffarian Tousi; Megen F. Velten; Timothy J. Bishop; Kelly K. Leong; Nicole S. Barkhordar; Grayson W. Marshall; Peter M. Loomer; Pranesh B. Aswath; Venu G. Varanasi;
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作者单位

Department of Biomedical Sciences, Baylor College of Dentistry, Texas A&M Health Science Center, Dallas, TX 75246, United States;

Department of Materials Science and Engineering, University of Texas at Arlington, Arlington, TX 75019, United States;

Division of Periodontology, School of Dentistry, University of California, San Francisco, CA 94143, United States;

Division of Periodontology, School of Dentistry, University of California, San Francisco, CA 94143, United States;

Department of Preventive and Restorative Dental Sciences, School of Dentistry, University of California, San Francisco, CA 94143, United States;

Department of Preventive and Restorative Dental Sciences, School of Dentistry, University of California, San Francisco, CA 94143, United States;

Division of Periodontology, School of Dentistry, University of California, San Francisco, CA 94143, United States;

Department of Materials Science and Engineering, University of Texas at Arlington, Arlington, TX 75019, United States;

Department of Biomedical Sciences, Baylor College of Dentistry, Texas A&M Health Science Center, Dallas, TX 75246, United States;

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正文语种 eng
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关键词
Bioactive glasses; Tissue engineering; Bone regeneration; Dental implant;

机译：生物活性玻璃;组织工程;骨再生;牙种植体;

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Combinatorial effect of Si~(4+), Ca~(2+), and Mg~(2+) released from bioactive glasses on osteoblast osteocalcin expression and biomineralization

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