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首页> 外文期刊>Materials science & engineering >Patterned carbon nanotubes as a new three-dimensional scaffold for mesenchymal stem cells
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Patterned carbon nanotubes as a new three-dimensional scaffold for mesenchymal stem cells

机译：图案化的碳纳米管作为间充质干细胞的新型三维支架

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摘要

We investigated the cellular adhesive features of mesenchymal stem cells (MSC) on non-coated and collagen coated patterned and vertically aligned carbon nanotube (CNT) structures mimicking the natural extra cellular matrix (ECM). Patterning was achieved using the elasto-capillary induced by water treatment on the CNT arrays. After confirmation with specific markers both at transcript and protein levels, MSCs from different passages were seeded on either collagen coated or non-coated patterned CNTs. Adhesion and growth of MSCs on the patterned CNT arrays were examined using scanning electron microscopy image analysis and 3-(4,5-dimethyl-2-thiazolyl)-2,5-diphenyl-tetrazolium bromide (MTT) assays. The highest MSC count was observed on the non-coated patterned CNTs at passage zero, while decreasing numbers of MSCs were found at the later passages. Similarly, MTT assay results also revealed a decrease in the viability of the MSCs for the later passages. Overall, the cell count and viability experiments indicated that MSCs were able to better attach to non-coated patterned CNTs compared to those coated with collagen. Therefore, the patterned CNT surfaces can be potentially used as a scaffold mimicking the ECM environment for MSC growth which presents an alternative approach to MSC-based transplantation therapy applications.
机译：我们研究了在非涂层和胶原蛋白涂层的图案化和垂直排列的碳纳米管（CNT）结构上的间充质干细胞（MSC）的细胞粘附特性，该结构模仿了自然的细胞外基质（ECM）。使用通过水处理在CNT阵列上诱导的弹性毛细管实现图案化。用转录物和蛋白质水平的特异性标记物确认后，将来自不同传代的MSC接种在胶原蛋白涂层或未涂层的图案化CNT上。使用扫描电子显微镜图像分析和3-（4,5-二甲基-2-噻唑基）-2,5-二苯基溴化四唑（MTT）分析法检查了MSC在图案化CNT阵列上的粘附和生长。在第0代时，在未涂覆的图案化CNT上观察到了最高的MSC计数，而在随后的几代中发现了数量减少的MSC。同样，MTT分析结果还显示，MSC在以后的传代中存活力下降。总的来说，细胞计数和生存力实验表明，与涂有胶原蛋白的那些相比，MSC能够更好地附着在未涂层的图案化CNT上。因此，图案化的CNT表面可以潜在地用作模仿ECM环境以生长MSC的支架，这为基于MSC的移植治疗应用提供了另一种方法。

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