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Effect of grain size on mechanical, surface and biological properties of microwave sintered hydroxyapatite

机译:粒度对微波烧结羟基磷灰石力学性能,表面性能和生物学性能的影响

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摘要

Hydroxyapatite (HA) compacts having average grain sizes of 168 ± 0.086 nm, 1.48 ± 0.627 μm and 5.01 ± 1.02 μm are processed from synthesized HA powder by microwave sintering at varying sintering temperature for different times. Superior mechanical and biological properties are shown by nano-grain HA compacts as compared to their micron grained counterparts. Compressive strength, indentation hardness, and indentation fracture toughness are increased with the decrease in HA grain size. The highest surface energy and maximum wettability are exhibited by nano-grain HA. HA compacts are assessed for cell-material interaction by SEM, MTT and immunochemistry assays using human osteoblast cell line for 1, 5 and 11 days. MTT assays showed higher number of living cells and faster proliferation on nano-grain HA surface. Osteoblast cells on nano-grain HA surface expressed significantly higher amount of vinculin and alkaline phosphatase (ALP) protein markers for cell adhesion and differentiation respectively. This study shows the effect of grain size on physical, mechanical and in vitro biological properties of microwave sintered HA compacts.
机译:通过在不同的烧结温度下以不同的时间进行微波烧结,从合成的HA粉中加工出平均粒径为168±0.086 nm,1.48±0.627μm和5.01±1.02μm的羟基磷灰石(HA)压块。纳米颗粒HA压块与微米颗粒的HA压块相比,具有优越的机械和生物学性能。随着HA晶粒尺寸的减小,抗压强度,压痕硬度和压痕断裂韧性增加。纳米颗粒HA表现出最高的表面能和最大的润湿性。使用人成骨细胞细胞系通过SEM,MTT和免疫化学分析评估HA压片的细胞-材料相互作用,持续1天,5天和11天。 MTT分析显示,纳米粒HA表面的活细胞数量更多,增殖更快。纳米粒HA表面上的成骨细胞分别表达了较高的纽蛋白和碱性磷酸酶(ALP)蛋白标记,分别用于细胞粘附和分化。这项研究表明晶粒尺寸对微波烧结HA压块的物理,机械和体外生物学特性的影响。

著录项

  • 来源
    《Materials science & engineering》 |2013年第5期|2846-2854|共9页
  • 作者单位

    W. M. Keck Biomedical Materials Research Laboratory, School of Mechanical and Materials Engineering, Washington State University, Pullman, WA 99164-2920, USA;

    W. M. Keck Biomedical Materials Research Laboratory, School of Mechanical and Materials Engineering, Washington State University, Pullman, WA 99164-2920, USA;

    W. M. Keck Biomedical Materials Research Laboratory, School of Mechanical and Materials Engineering, Washington State University, Pullman, WA 99164-2920, USA;

    W. M. Keck Biomedical Materials Research Laboratory, School of Mechanical and Materials Engineering, Washington State University, Pullman, WA 99164-2920, USA;

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  • 原文格式 PDF
  • 正文语种 eng
  • 中图分类
  • 关键词

    Nanostructure; Microwave sintering; Grain size; Hydroxyapatite; Mechanical property; Vinculin and ALP expression;

    机译:纳米结构微波烧结;晶粒大小;羟基磷灰石;机械性能Vinculin和ALP表达;

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