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In vitro and in vivo evaluation of chitosan-gelatin scaffolds for cartilage tissue engineering

机译：壳聚糖-明胶支架在软骨组织工程中的体外和体内评价

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摘要

Chitosan-gelatin polyelectrolyte complexes were fabricated and evaluated as tissue engineering scaffolds for cartilage regeneration in vitro and in vivo. The crosslinker for the gelatin component was selected among glutar-aldehyde, bisepoxy, and a water-soluble carbodiimide (WSC) based upon the proliferation of chondrocytes on the crosslinked gelatin. WSC was found to be the most suitable crosslinker. Complex scaffolds made from chito-san and gelatin with a component ratio equal to one possessed the proper degradation rate and mechanical stability in vitro. Chondrocytes were able to proliferate well and secrete abundant extracellular matrix in the chitosan-gelatin (1:1) complex scaffolds crosslinked by WSC (C1G1_(wsc)) compared to the non-crosslinked scaffolds. Implantation of chondrocytes-seeded scaffolds in the defects of rabbit articular cartilage confirmed that C1G2_(wsc) promoted the cartilage regeneration. The neotissue formed the histological feature of tide line and lacunae in 6.5 months. The amount of glycosaminoglycans in C1G1_(wsc) constructs (0.187 ± 0.095 μg/mg tissue) harvested from the animals after 6.5 months was 14 wt.% of that in normal cartilage (1.329 ± 0.660 μg/mg tissue). The average compressive modulus of regenerated tissue at 6.5 months was about 0.539 MPa, which approached to that of normal cartilage (0.735 MPa), while that in the blank control (3.881 MPa) was much higher and typical for fibrous tissue. Type Ⅱ collagen expression in C1G1_(wsc) constructs was similarly intense as that in the normal hyaline cartilage. According to the above results, the use of C1G1_(wsc) scaffolds may enhance the cartilage regeneration in vitro and in vivo.
机译：制备了壳聚糖-明胶聚电解质复合物，并将其评估为用于体外和体内软骨再生的组织工程支架。基于软骨细胞在交联明胶上的增殖，从戊二醛，双环氧和水溶性碳二亚胺（WSC）中选择明胶组分的交联剂。发现WSC是最合适的交联剂。由壳聚糖和明胶制成的复合支架，其组分比等于1，在体外具有适当的降解速率和机械稳定性。与未交联的支架相比，软骨细胞能够很好地增殖并在通过WSC（C1G1_（wsc））交联的壳聚糖-明胶（1：1）复合支架中分泌丰富的细胞外基质。在兔关节软骨缺损中植入软骨细胞种植的支架证实了C1G2_（wsc）促进了软骨的再生。在6.5个月内，新组织形成了潮线和凹陷的组织学特征。在6.5个月后从动物中收获的C1G1_（wsc）构建体（0.187±0.095μg/ mg组织）中糖胺聚糖的量为正常软骨（1.329±0.660μg/ mg组织）中的14 wt。％。再生组织在6.5个月时的平均压缩模量约为0.539 MPa，接近正常软骨的平均压缩模量（0.735 MPa），而空白对照组的平均压缩模量（3.881 MPa）更高，并且是纤维组织的典型值。 C1G1_（wsc）构建物中的Ⅱ型胶原表达与正常透明软骨相似。根据以上结果，使用C1G1_（wsc）支架可以增强体外和体内的软骨再生。

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  • 来源
    《Materials science & engineering》 |2013年第5期|2855-2863|共9页
  • 作者

    Shu Wen Whu; Kun-Che Hung; Kuo-Huang Hsieh; Chih-Hwa Chen; Ching-Lin Tsai; Shan-hui Hsu;

  • 作者单位

    Department of Orthopaedic Surgery, Chang Gung Memorial Hospital at Keelung, College of Medicine, Chang Gung University, Taoyuan, Taiwan;

    Institute of Polymer Science and Engineering, National Taiwan University, Taipei, Taiwan;

    Institute of Polymer Science and Engineering, National Taiwan University, Taipei, Taiwan;

    Department of Orthopaedic Surgery, Chang Gung Memorial Hospital at Keelung, College of Medicine, Chang Gung University, Taoyuan, Taiwan;

    Department of Orthopaedics, National Taiwan University Hospital, No. 1,Sec, 1,Jen Ai Rd,,Taipei 10051,Taiwan;

    Institute of Polymer Science and Engineering, National TaiwanUniversity,No,1,Sec. 4,Roosevelt Rd,, Taipei 10617, Taiwan, 10617;

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  • 正文语种 eng
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  • 关键词

    Chitosan; Gelatin; Scaffold; Tissue engineering; Hyaline cartilage regeneration;

    机译：壳聚糖明胶;脚手架;组织工程;透明软骨再生;

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