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首页> 外文期刊>Materials science & engineering >Injectable gellan gum/lignocellulose nanofibrils hydrogels enriched with melatonin loaded forsterite nanoparticles for cartilage tissue engineering: Fabrication, characterization and cell culture studies
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Injectable gellan gum/lignocellulose nanofibrils hydrogels enriched with melatonin loaded forsterite nanoparticles for cartilage tissue engineering: Fabrication, characterization and cell culture studies

机译：可注射的Gellan Gum / Lignocellulose纳米纤维水凝胶用褪黑素负载的甜菜素纳米粒子用于软骨组织工程：制造，表征和细胞培养研究

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Injectable hydrogels based on natural polysaccharides have attracted considerable attention in cartilage tissue engineering, especially those reinforced with mineral nanofilers carrying drug molecules. Here, a novel injectable hydrogel based on gellan gum (GG)/lignocellulose nanofibrils (LG(NF)) composite enriched with melatonin (MEL) loaded forsterite (FS) nanoparticles (FS-MEL) was developed to yield enhanced mechanical and biological properties of the hydrogels. Gelation time and temperature were determined for different hydrogel formulation containing 1-5 w/v% LG(NF) and 0.1-0.3 w/v% FS-MEL. The injectability WA proved the ease of injection of the developed hydrogels. Degradation rate and swelling degree of developed hydrogel were evaluated to determine the effect of LG(NF) and FS on hydrogel behaviour. Results of mechanical characterization showed that the compressive modulus and strength of GG hydrogels were improved by incorporation of LG(NF) and FS. The results of MEL release study in PBS revealed that MEL showed more sustained release from the hydrogel compared to FS nanoparticles. Cell-hydrogels interaction was evaluated by culturing chondrocyte cells. Results exhibited higher cell adhesion, proliferation and gene expression on GG/LG(NF)/FS-MEL hydrogel compared to GG/LG(NF) and GG/LG(NF)/FS, which can be attributed to the synergic effect of FS and MEL. Overall results demonstrated that the developed GG/LG(NF)/FS-MEL hydrogels can be offered as promising materials for cartilage regeneration applications.
机译：基于天然多糖的可注射水凝胶在软骨组织工程中引起了相当大的关注，特别是那些用携带药物分子的矿物纳米膜增强的那些。此处，基于Gellan Gul胶（GG）/木质纤维素纳米纤维（Lg（NF））复合材料的新型可注射水凝胶（Lg（NF））复合材料富含褪黑素（MEL）纳米粒子（FS）纳米颗粒（FS-MEL），以产生增强的机械和生物学性质水凝胶。确定含有1-5w / v％Lg（NF）和0.1-0.3W / v％FS-MEL的不同水凝胶制剂的凝胶化时间和温度。可注射性WA证明了发育水凝胶的易用性。评估发育水凝胶的降解速率和溶胀度以确定LG（NF）和FS对水凝胶行为的影响。机械表征的结果表明，通过掺入LG（NF）和FS，改善了GG水凝胶的压缩模量和强度。与FS纳米颗粒相比，PBS中MEL释放研究的结果表明，与水凝胶相比，MEL显示出更持续释放。通过培养软骨细胞细胞评估细胞 - 水凝胶相互作用。结果表现出与GG / Lg（NF）和GG / LG（NF）/ FS相比的GG / Lg（NF）/ FS-MEL水凝胶上的更高细胞粘附，增殖和基因表达，其可归因于FS的协同作用和梅尔。总体结果证明，开发的GG / LG（NF）/ FS-MEL水凝胶作为软骨再生应用的有希望的材料。

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