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首页> 外文期刊>Materials science & engineering >Osteoblastic exosomes. A non-destructive quantitative approach of alkaline phosphatase to assess osteoconductive nanomaterials
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Osteoblastic exosomes. A non-destructive quantitative approach of alkaline phosphatase to assess osteoconductive nanomaterials

机译：骨细胞外泌体。碱性磷酸酶的非破坏性定量方法评估骨导电纳米材料

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摘要

Alkaline phosphatase (ALP) is an essential biomarker of osteoblastic activity. Currently, ALP activity has been used to study bone mineralization mechanisms and osteoactive biomaterials among others. The ALP quantification is usually performed by destructive methods either on growing cells or cells lysate in which the osteoconductive biomaterial is being assessed. This work addresses the evaluation of a non-destructive colorimetric approach for the determination of ALP activity on osteoblast-derived exosomes from culture supernatants. The efficiency of the method was evaluated on osteoconductive electrospun scaffolds of PCL compounded with ZnO as a reference biomaterial. The results demonstrated that the osteoblast cell line mineralization induced by osteoconductive scaffolds can be monitorized over time by the non-destructive measurement of ALP activity on osteoblast derived exosomes. Consequently, this non-destructive approach suggested to be a reliable alternative technique for the quantification of biomaterials osteoconductivity or even evaluation of osteoblastic response at stem cells.
机译：碱性磷酸酶（ALP）是骨细胞活性的必要生物标志物。目前，ALP活性已被用于研究骨矿化机制和骨质活性生物材料等。 ALP定量通常通过破坏性方法进行，无论是在生长细胞还是裂解物中，其中都会被评估骨导电性生物材料的裂解物。该工作解决了从培养上清液中测定植物细胞衍生的外泌体上的非破坏性比色方法的评价。评价该方法的效率在用ZnO为参考生物材料的PCL配混的骨导电性电纺支架。结果表明，通过对骨盆衍生出外索体的非破坏性测量，可以随时间监测骨细胞诱导的骨细胞细胞系矿化。因此，这种非破坏性方法表明是一种可靠的替代技术，用于定量生物材料骨导电性甚至对干细胞的骨细胞响应的评估。

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  • 来源
    《Materials science & engineering》 |2020年第10期|110931.1-110931.9|共9页
  • 作者

    Alejandra Sanchez M.; Felice Betiana; Sappia Luciano D.; Lima Moura Silio; Marti Merce; Isabel Pividori M.;

  • 作者单位

    UNT Inst Super Invest Biol INSIBIO CONICET Dept Bioingn Lab Medios & Interfases Fac Ciencias Exactas & Te Av Independencia 1800 RA-4000 San Miguel De Tucuman Tucuman Argentina|Univ Autonoma Barcelona Dept Quim Grp Sensors & Biosensors Bellaterra 08193 Spain;

    UNT Inst Super Invest Biol INSIBIO CONICET Dept Bioingn Lab Medios & Interfases Fac Ciencias Exactas & Te Av Independencia 1800 RA-4000 San Miguel De Tucuman Tucuman Argentina|Univ Autonoma Barcelona Dept Quim Grp Sensors & Biosensors Bellaterra 08193 Spain;

    UNT Inst Super Invest Biol INSIBIO CONICET Dept Bioingn Lab Medios & Interfases Fac Ciencias Exactas & Te Av Independencia 1800 RA-4000 San Miguel De Tucuman Tucuman Argentina|Univ Autonoma Barcelona Dept Quim Grp Sensors & Biosensors Bellaterra 08193 Spain|Univ Nacl La Plata Inst Invest Fisicoquim Teor & Aplicadas INIFTA Fac Cs Exactas CONICET 64 & Diag 113 RA-1900 La Plata Argentina;

    Univ Autonoma Barcelona Dept Quim Grp Sensors & Biosensors Bellaterra 08193 Spain;

    Univ Autonoma Barcelona Dept Quim Grp Sensors & Biosensors Bellaterra 08193 Spain;

    Univ Autonoma Barcelona Dept Quim Grp Sensors & Biosensors Bellaterra 08193 Spain;

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  • 正文语种 eng
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  • 关键词

    Alkaline phosphatase; Exosomes; Osteoblasts cell line; Biomaterial characterization; Biomarker; Bone tissue engineering;

    机译：碱性磷酸酶;外泌体;成骨细胞系;生物材料表征;生物标志物;骨组织工程;

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