...
  • 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Materials science & engineering >Fabrication of a sensitive label free electrochemical immunosensor for detection of prostate specific antigen using functionalized multi-walled carbon nanotubes/polyaniline/AuNPs
【24h】

Fabrication of a sensitive label free electrochemical immunosensor for detection of prostate specific antigen using functionalized multi-walled carbon nanotubes/polyaniline/AuNPs

机译：使用官能化多壁碳纳米管/聚苯胺/ AUNPS检测前列腺特异性抗原的敏感标记电化学免疫传感器的制备

获取原文
获取原文并翻译 | 示例
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

The aim of this research is to introduce a novel label free electrochemical immunosensor based on glassy carbon electrode (GCE) modified with carboxylated carbon nanotubes (COOH-MWCNTs)/polyaniline (PANI)/gold nanoparticles (AuNPs) for the detection of prostate specific antigen (PSA). The AuNPs were utilized as a connector for PSA antibody immobilization through NH2 groups on antibody. Investigations on modified electrode surface were performed by FT-IR spectrum, scanning electron microscope (SEM) and energy dispersive X-ray spectroscopy (EDS) to evaluate the synthesized nanocomposite and modified electrode surface. As a sensitive analytical method for the detection of PSA, differential pulse voltammetry (DPV) was employed in different ranges of antigen concentration, 1.66 ag.mL(-1) to 1.3 ngmL(-1). In addition, the detection limit was obtained 0.5 pg.mL(-1), from the linear relationship between antigen concentration log and peak current. Also, the proposed immunosensor was carried out for the determination of PSA in human serum samples, indicating recoveries ranging from 92 to 104%. Finally, it should be noted that the reproducibility and specificity, along with the stability of the present immunosensor were examined, and satisfactory findings were obtained, thus proving it as a promising PSA immunosensor.
机译：本研究的目的是基于用羧化碳纳米管（CoOH-MWCNT）/聚苯胺（PANI）/金纳米胺（AUNP）改性的玻璃碳电极（GCE）引入一种新型标记的游离电化学免疫传感器，用于检测前列腺特异性抗原（PSA）。 AUNPS用作PSA抗体固定通过NH 2在抗体上的连接器的连接器。通过FT-IR光谱，扫描电子显微镜（SEM）和能量分散X射线光谱（EDS）进行对改性电极表面的研究以评估合成的纳米复合材料和改性电极表面。作为检测PSA的敏感性分析方法，使用差分脉冲伏安法（DPV）在不同范围的抗原浓度范围内，1.66％Ag.ML（-1）至1.3 ngml（-1）。此外，从抗原浓度对数和峰值电流之间的线性关系获得0.5pg.ml（-1）的检出限。此外，所提出的免疫传感器是为了测定人血清样品中PSA，表明回收率为92-104％。最后，应注意，研究了再现性和特异性以及对本发明免疫传感器的稳定性进行了检查，获得了令人满意的结果，从而证明其作为有前途的PSA免疫传感器。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号