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首页> 外文期刊>Materials science & engineering >Immunosensors containing solution blow spun fibers of poly(lactic acid) to detect p53 biomarker
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Immunosensors containing solution blow spun fibers of poly(lactic acid) to detect p53 biomarker

机译：含有聚（乳酸）的溶液吹纺纤维的免疫传感器以检测P53生物标志物

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摘要

This paper reports on biosensors made with a matrix of polylactic acid (PLA) fibers, which are suitable for immobilization of the anti-p53 active layer for detection of p53 biomarker. The PLA fibers were produced with solution blow spinning, a method that is advantageous for its simplicity and possibility to tune the fiber properties. For the biosensors, the optimized time to deposit the fibers was 60 s, with which detection of p53 could be achieved with the limit of detection of 11 pg/mL using electrical impedance spectroscopy. This sensitivity is also sufficient to detect the p53 biomarker in patient samples, which was confirmed by distinguishing samples from cell lines with distinct p53 concentrations in a plot where the impedance spectra were visualized with the interactive document mapping (IDMAP) technique. The high sensitivity and selectivity of the biosensors may be attributed to the specific interaction between the active layer and p53 modeled with a Langmuir-Freundlich and Freundlich isotherms and inferred from the analysis of the vibrational bands at 1550, 1650 and 1757 cm(-1) using polarization-modulated infrared reflection absorption spectroscopy (PM-IRRAS). The successful immobilization of the active layer is evidence that the approach based on solution blown spun fibers may be replicated to other types of biosensors.
机译：本文报告了用聚乳酸（PLA）纤维基质制备的生物传感器，其适用于固定抗P53活性层以检测P53生物标志物。通过溶液吹纺生产PLA纤维，这是一种有利于其简单性和曲调纤维性能的方法。对于生物传感器，沉积纤维的优化时间为60秒，可以通过使用电阻抗光谱检测11pg / ml的限制来实现P53的检测。这种敏感性也足以检测患者样品中的p53生物标志物，其通过将来自细胞系的样品与不同的P53浓度区分地区的曲线中与交互式文档映射（IDMAP）技术可视化的曲线中的不同P53浓度来确认。生物传感器的高灵敏度和选择性可以归因于用Langmuir-Freundlich和Freundlich等温线建模的有源层和P53之间的特定相互作用，并从1550,1650和1757cm（-1）的振动带的分析推断出来使用偏振调制的红外反射吸收光谱（PM-IRRAS）。活性层的成功固定是证据表明，基于溶液吹扫纤维的方法可以复制到其他类型的生物传感器。

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  • 来源
    《Materials science & engineering》 |2020年第10期|111120.1-111120.11|共11页
  • 作者

    Soares Andrey Coatrini; Soares Juliana Coatrini; Paschoalin Rafaella Takehara; Rodrigues Valquiria Cruz; Melendez Matias Eliseo; Reis Rui M.; Carvalho Andre Lopes; Capparelli Mattoso Luiz Henrique; Oliveira Jr Osvaldo N.;

  • 作者单位

    Embrapa Instrumentat Nanotechnol Natl Lab Agribusiness LNNA BR-13560970 Sao Carlos SP Brazil|Univ Sao Paulo Sao Carlos Inst Phys BR-13566590 Sao Carlos Brazil;

    Embrapa Instrumentat Nanotechnol Natl Lab Agribusiness LNNA BR-13560970 Sao Carlos SP Brazil|Univ Sao Paulo Sao Carlos Inst Phys BR-13566590 Sao Carlos Brazil;

    Univ Sao Paulo Sao Carlos Inst Phys BR-13566590 Sao Carlos Brazil;

    Univ Sao Paulo Sao Carlos Inst Phys BR-13566590 Sao Carlos Brazil;

    Barretos Canc Hosp Mol Oncol Res Ctr BR-14784400 Barretos Brazil|Little Prince Coll Pele Little Prince Res Inst Little Prince Complex Curitiba BR-80250060 Curitiba Parana Brazil;

    Barretos Canc Hosp Mol Oncol Res Ctr BR-14784400 Barretos Brazil|Univ Minho Sch Med Life & Hlth Sci Res Inst ICVS P-4710057 Braga Portugal|ICVS 3Bs PT Govt Associate Lab P-4805017 Braga Portugal;

    Barretos Canc Hosp Mol Oncol Res Ctr BR-14784400 Barretos Brazil;

    Embrapa Instrumentat Nanotechnol Natl Lab Agribusiness LNNA BR-13560970 Sao Carlos SP Brazil|Univ Fed Sao Carlos Mat Engn Dept BR-13565905 Sao Carlos SP Brazil;

    Univ Sao Paulo Sao Carlos Inst Phys BR-13566590 Sao Carlos Brazil;

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  • 正文语种 eng
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  • 关键词

    PLA fibers; Solution blow spinning; Immunosensor; Cancer; p53;

    机译：PLA纤维;溶液吹纺;免疫传感器;癌症;P53;

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