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首页> 外文期刊>Materials science & engineering >Fish protein hydrolysate production from sardine solid waste by crude pepsin enzymatic hydrolysis in a bioreactor coupled to an ultrafiltration unit
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Fish protein hydrolysate production from sardine solid waste by crude pepsin enzymatic hydrolysis in a bioreactor coupled to an ultrafiltration unit

机译：在与超滤装置相连的生物反应器中，通过胃蛋白酶的粗胃蛋白酶水解从沙丁鱼固体废物中生产鱼蛋白

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摘要

The aims of the study were to optimize the production a fish protein hydrolysate (FPH) by enzymatic hydrolysis of sardine solid waste using crude pepsin, and to scale up the process in a bioreactor coupled to an ultrafiltration unit for product recovery. Results showed that the crude pepsin prepared by autolysis of the mucous membranes of a sheep stomach at optimal conditions (i. e. pH = 1.5-2 and incubation time of 6 h) could be satisfactory used for the enzymatic hydrolysis offish solid waste. The optimal conditions for enzymatic reaction were: temperature 48 ℃, and pH 1.5. The scale up of the enzymatic hydrolysis and the coupling of the reactor an ultrafiltration unit to concentrate the hydrolysate gave good results with a rejection coefficient for the protein hydrolysate product in the range of 90%. The volumetric concentration factor was 2.5, with a permeate flux of 200 L m~(-2) bar~(-1). However, the results also suggest that the ultrafiltration product concentration process may be operating beyond the critical flux at which point irreversible membrane fouling occurs.
机译：该研究的目的是通过使用粗胃蛋白酶对沙丁鱼固体废物进行酶促水解来优化鱼蛋白水解产物（FPH）的生产，并在与超滤单元相连的生物反应器中扩大该过程的规模，以回收产品。结果表明，在最佳条件下（即pH ＝ 1.5-2，温育时间为6小时）通过羊胃粘膜的自溶制备的粗胃蛋白酶可以令人满意地用于酶水解鱼固体废物。酶促反应的最佳条件为：温度48℃，pH 1.5。酶水解的放大和反应器与超滤单元的耦合以浓缩水解产物产生了良好的结果，蛋白质水解产物的排斥系数在90％的范围内。体积浓度因子为2.5，渗透通量为200 L m〜（-2）bar〜（-1）。但是，结果还表明，超滤产物浓缩过程可能会超出临界流量，在临界流量处发生不可逆的膜污染。

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  • 来源
    《Materials science & engineering》 |2012年第4期|p.922-928|共7页
  • 作者

    M.S. Benhabiles; N. Abdi; N. Drouiche; H. Lounici; A. Pauss; M.F.A. Goosen; N. Mameri;

  • 作者单位

    National Polytechnic school of Algiers, B.P. 182-16200, El Harrach, Algiers, Algeria;

    National Polytechnic school of Algiers, B.P. 182-16200, El Harrach, Algiers, Algeria;

    National Polytechnic school of Algiers, B.P. 182-16200, El Harrach, Algiers, Algeria,Silicon Technology Development Unit (UDTS) 2, Bd Frantz Fanon BPUO, Alger-7 Merveilles, 16000, Algeria,Silicon Technology Development Unit, Department of Environmental Engineering, 2, Bd Frantz Fanon BP140 Alger-7-mervielles Alger 16000, Algeria;

    National Polytechnic school of Algiers, B.P. 182-16200, El Harrach, Algiers, Algeria;

    University of Technology of Compiegne, Departement Genie chimique.B.P. 20.509, 60205 Compiegne cedex, France;

    Alfaisal University, Riyadh, Saudi Arabia;

    University of Technology of Compiegne, Departement Genie chimique.B.P. 20.509, 60205 Compiegne cedex, France;

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  • 原文格式 PDF
  • 正文语种 eng
  • 中图分类
  • 关键词

    enzyme; protein hydrolysate; ultrafiltration; bioreactor; biochemical engineering; food processing;

    机译：酶蛋白质水解物超滤生物反应器生化工程;食品加工;

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