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首页> 外文期刊>Materials science & engineering >Osteoblast-like cell ingrowth, adhesion and proliferation on porous Ti-6Al-4V with particulate and fiber scaffolds
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Osteoblast-like cell ingrowth, adhesion and proliferation on porous Ti-6Al-4V with particulate and fiber scaffolds

机译：带有颗粒和纤维支架的多孔Ti-6Al-4V上成骨细胞样细胞向内生长，粘附和增殖

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摘要

This paper presents the results of an experimental study of osteoblast-like cell ingrowth into porous Ti-6Al-4V structures with well-controlled geometries. The effects of pore size and strut geometry are elucidated in in-vitro cell ingrowth experiments on porous Ti-6Al-4V structures with particulate and fiber geometries. The initial stages of cell spreading and proliferation are examined using cell culture experiments. Scanning electron microscope (SEM) and a methylthiazol tetrazolium (MTT) assay are used to reveal the initial stages of cell spreading and attachment. Enzymatic detachment tests are also used to examine cell adhesion after 48 h of cell culture. The results show a strong effect of pore size on the rate of cell bridging over gaps. The extent of cell ingrowth, initial cell adhesion and cell proliferation also increase with decreasing pore size. A lower incidence of cell bridging (over gaps) is observed on the fiber porous structures. However, fiber geometries enable contact guidance during cell spreading along the fiber directions. This enhances the extent of cell ingrowth into the fiber porous structures. No significant differences are observed in cell adhesion and proliferation on porous structures with similar pore sizes.
机译：本文介绍了成骨细胞样细胞向内生长到几何形状可控的多孔Ti-6Al-4V结构中的实验研究结果。在具有颗粒和纤维几何形状的多孔Ti-6Al-4V结构的体外细胞向内生长实验中阐明了孔径和支杆几何形状的影响。使用细胞培养实验检查细胞扩散和增殖的初始阶段。扫描电子显微镜（SEM）和甲基噻唑四唑（MTT）分析用于揭示细胞扩散和附着的初始阶段。在细胞培养48小时后，还使用酶分离试验来检查细胞粘附。结果显示，孔径对间隙上细胞桥接的速率有很大影响。细胞向内生长的程度，初始细胞粘附和细胞增殖也随着孔径的减小而增加。在纤维多孔结构上观察到细胞桥接（间隙）的发生率较低。但是，纤维的几何形状可以在细胞沿纤维方向扩散期间引导接触。这增加了细胞向纤维多孔结构中的向内生长的程度。在具有相似孔径的多孔结构上，细胞粘附和增殖方面未观察到显着差异。

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  • 来源
    《Materials science & engineering》 |2010年第5期|p.647-656|共10页
  • 作者

    Jianbo Chen; Emily Paetzell; Jikou Zhou; Lauren Lyons; Wole Soboyejo;

  • 作者单位

    Princeton Institute for Science and Technology of Materials (PRISM), Department of Mechanical and Aerospace Engineering, Princeton University, Princeton, NJ 08544, United States;

    Princeton Institute for Science and Technology of Materials (PRISM), Department of Mechanical and Aerospace Engineering, Princeton University, Princeton, NJ 08544, United States;

    NanoSteel Company, Idaho Falls, ID 83402, United States;

    Princeton Institute for Science and Technology of Materials (PRISM), Department of Mechanical and Aerospace Engineering, Princeton University, Princeton, NJ 08544, United States;

    Princeton Institute for Science and Technology of Materials (PRISM), Department of Mechanical and Aerospace Engineering, Princeton University, Princeton, NJ 08544, United States;

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  • 原文格式 PDF
  • 正文语种 eng
  • 中图分类
  • 关键词

    porous Ti-6Al-4V structures; particulates; fibers; cell ingrowth; proliferation; adhesion;

    机译：多孔的Ti-6Al-4V结构;颗粒纤维;细胞向内生长增殖;附着力;

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