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首页> 外文期刊>Materials science & engineering >Boosting transfection efficiency: A systematic study using layer-by-layer based gene delivery platform
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Boosting transfection efficiency: A systematic study using layer-by-layer based gene delivery platform

机译：促进转染效率：使用基于层基的基因递送平台进行系统研究

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摘要

Nowadays, the nanoparticle-based delivery approach is becoming more and more attractive in gene therapy due to its low toxicity and immunogenicity, sufficient packaging capacity, targeting, and straightforward, low-cost, large-scale good manufacturing practice (GMP) production. A number of research works focusing on multilayer structures have explored different factors and parameters that can affect the delivery efficiency of pDNA. However, there are no systematic studies on the performance of these structures for enhanced gene delivery regarding the gene loading methods, the use of additional organic components and cell/particle incubation conditions. Here, we conducted a detailed analysis of different parameters such as (i) strategy for loading pDNA into carriers, (ii) incorporating both pDNA and organic additives within one carrier and (iii) variation of cell/ particle incubation conditions, to evaluate their influence on the efficiency of pDNA delivery with multilayer structures consisting of inorganic cores and polymer layers. Our results reveal that an appropriate combination of all these parameters leads to the development of optimized protocols for high transfection efficiency, compared to the non-optimized process ( 70% vs. 7%), and shows a good safety profile. In conclusion, we provide the proof-of-principle that these multilayer structures with the developed parameters are a promising non-viral platform for an efficient delivery of nucleic acids.
机译：如今，基于纳米粒子的递送方法由于其低毒性和免疫原性，足够的包装能力，靶向，直接，低成本，大规模的良好制造实践（GMP）生产而在基因治疗中变得越来越有吸引力。专注于多层结构的许多研究工作探索了可能影响PDNA的输送效率的不同因素和参数。然而，没有关于这些结构的性能的系统研究，用于增强关于基因负载方法的基因递送，使用额外的有机组分和细胞/颗粒孵育条件。在这里，我们对不同参数进行了详细的分析，例如（i）将PDNA加载到载体中的策略，（ii）将PDNA和有机添加剂掺入（III）细胞/颗粒孵育条件的变异中，以评估它们的影响用由无机芯和聚合物层组成的多层结构PDNA递送的效率。我们的结果表明，与非优化过程（70％与7％）相比，所有这些参数的适当组合导致所有这些参数的优化方案用于高转染效率的优化方案，并显示出良好的安全性。总之，我们提供了原则上，这些多层结构具有发达参数是有效的非病毒平台，用于有效地递送核酸。

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  • 来源
    《Materials science & engineering》 |2021年第7期|112161.1-112161.11|共11页
  • 作者

    Tarakanchikova Yana V.; Linnik Dmitrii S.; Mashel Tatiana; Muslimov Albert R.; Pavlov Sergey; Lepik Kirill V.; Zyuzin Mikhail V.; Sukhorukov Gleb B.; Timin Alexander S.;

  • 作者单位

    Peter Great St Petersburg Polytech Univ Polytech Skaya 29 St Petersburg 195251 Russia|St Petersburg Acad Univ Nanobiotechnol Lab St Petersburg 194021 Russia;

    Peter Great St Petersburg Polytech Univ Polytech Skaya 29 St Petersburg 195251 Russia;

    Peter Great St Petersburg Polytech Univ Polytech Skaya 29 St Petersburg 195251 Russia|ITMO Univ Dept Appl Opt Kronverkskiy Pr 49 St Petersburg 197101 Russia;

    St Petersburg Acad Univ Nanobiotechnol Lab St Petersburg 194021 Russia;

    Ioffe Inst Politekh Skaya Ulitsa 26 St Petersburg 194021 Russia;

    Pavlov Univ RM Gorbacheva Res Inst Pediat Oncol Hematol & Tra Lev Tolstoy Str 6-8 St Petersburg 197022 Russia;

    ITMO Univ Dept Phys & Engn Lomonosova 9 St Petersburg 191002 Russia;

    Skolkovo Inst Sci & Technol Moscow 143026 Russia|Queen Mary Univ London Sch Engn & Mat Sci London England;

    Peter Great St Petersburg Polytech Univ Polytech Skaya 29 St Petersburg 195251 Russia|Natl Res Tomsk Polytech Univ Lenin Ave 30 Tomsk 634050 Russia;

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  • 正文语种 eng
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  • 关键词

    Core-shell particles; Gene delivery; Calcium carbonate; Organic additives; DNase inhibitor; Cell incubation protocol;

    机译：核 - 壳颗粒;基因递送;碳酸钙;有机添加剂;DNase抑制剂;细胞孵化协议;

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