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首页> 外文期刊>Materials science & engineering >An electro-conductive hybrid scaffold as an artificial Bruch's membrane
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An electro-conductive hybrid scaffold as an artificial Bruch's membrane

机译：作为人造Bruch膜的电导电杂交脚手架

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Many research groups have investigated the various kinds of scaffolds to mimic the natural Bruch's membrane (BM) and support the retinal pigmented epithelial cells to form an organized cellular monolayer. While using prosthetic BM is identified as a promising treatment of age-related macular degeneration (AMD), a degenerative and progressive retinal disease, the effects of different signals such as electrical and morphological cues on the retinal pigmented epithelial (RPE) cells are still unknown. In this study, a laminated and conductive hydrogel/ fiber composite scaffold by adding conductive polyaniline (PANi) to the scaffold's nanofibrous phase was prepared. This hybrid scaffold offers the closest morphology to the native structure of the human Bruch's membrane by imitating the inner and outer collagenous layer and induces the electrical signal to the scaffold to assess the electrical cue on behaviors of polarized retinal pigmented epithelial cells in the retina. The electrospun nanofibrous phase consisted of gelatin-Polyaniline in different ratios incorporated into the hydrogel precursor, a blend of gelatin and 4-armed PEG. We used a novel dual crosslinking process by incorporating the exposure of gamma irradiation and glutaraldehyde vapor treatment to construct the scaffold's hydrogel phase. The results showed the best composition was the sample which included the 40/60, Polyaniline/gelatin nanofiber sheets ratio because this scaffold revealed a 2.66 +/- 0.33 MPa, Young's modulus and 1.84 +/- 0.21 S/cm, electrochemical conductivity, which are close to the main features of native Bruch's membrane. In addition, this scaffold showed good biocompatibility by reaching 83.47% cell viability.
机译：许多研究组研究了各种支架以模拟天然Bruch的膜（BM）并支持视网膜着色的上皮细胞以形成有组织的细胞单层。在使用假体BM的同时被鉴定为具有年龄相关的黄斑变性（AMD）的有前途的治疗，因此退行性和渐进式视网膜疾病，不同信号的影响如电力和形态线索上的视网膜色素上皮（RPE）细胞仍然未知。在该研究中，制备通过将导电聚苯胺（PANI）添加到支架纳米纤维相中的层压和导电水凝胶/纤维复合支架。这种混合件通过模仿内部和外胶原层对人Bruch膜的天然结构提供最接近的形态，并诱导支架的电信号，以评估视网膜中偏振视网膜色素上皮细胞的行为的电气提示。电纺纳米纤维相由掺入水凝胶前体的不同比例的明胶 - 聚苯胺组成，包括明胶和4臂佩格的混合物。我们通过掺入γ辐射和戊二醛蒸气处理来构建支架水凝胶相的新型双交联过程。结果表明，最佳的组合物是包含40/60，聚苯胺/明胶纳米纤维片材的样品，因为该支架显示出2.66 +/- 0.33MPa，杨氏模量和1.84 +/- 0.21 s / cm，电化学电导率接近天然Bruch膜的主要特征。此外，该支架通过达到83.47％的细胞活力显示出良好的生物相容性。

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