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首页> 外文期刊>Materials science & engineering >Biocompatible polypeptide nanogel: Effect of surfactants on nanogelation in inverse miniemulsion, in vivo biodistribution and blood clearance evaluation
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Biocompatible polypeptide nanogel: Effect of surfactants on nanogelation in inverse miniemulsion, in vivo biodistribution and blood clearance evaluation

机译：生物相容性多肽纳米凝胶：体内生物分布和血液清除评估中纳米晶体中表面活性剂对纳米晶体的影响

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摘要

Horseradish peroxidase (HRP)/H2O2-mediated crosslinking of polypeptides in inverse miniemulsion is a promising approach for the development of next-generation biocompatible and biodegradable nanogels. Herein, we present a fundamental investigation of the effects of three surfactants and their different concentrations on the (HRP)/H2O2-mediated nanogelation of poly[N5-(2-hydroxyethyl)-L-glutamine-ran-N5-propargyl-L-glutamine-ranN5-(6-aminohexyl)-L-glutamine]-ran-N5-[2-(4-hydroxyphenyl)ethyl)-L-glutamine] (PHEG-Tyr) in inverse miniemulsion. The surfactants sorbitan monooleate (SPAN 80), polyoxyethylenesorbitan trioleate (TWEEN 85), and dioctyl sulfosuccinate sodium salt (AOT) were selected and their influence on the nanogel size, size distribution, and morphology was evaluated. The most effective nanogelation stabilization was achieved with 20 wt% nonionic surfactant SPAN 80. The diameter of the hydrogel nanoparticles was 230 nm (dynamic light scattering, DLS) and was confirmed also by nanoparticle tracking analysis (NTA) which showed the diameters ranging from 200 to 300 nm. Microscopy and image analyses showed that the nanogel in the dry state was spherical in shape and had number-average diameter Dn = 26 nm and dispersity SIC = 1.91. In the frozen-hydrated state, the nanogel appeared porous and was larger in size with Dn = 182 nm and SIC = 1.52. Our results indicated that the nanogelation of the polymer precursor required a higher concentration of surfactant than classical inverse miniemulsion polymerization to ensure effective stabilization. The developed polypeptide nanogel was radiolabeled with 125I, and in vivo biodistribution and blood clearance evaluations were performed. We found that the 125Ilabeled nanogel was well-biodistributed in the bloodstream, cleared from mouse blood during 48 h by renal and hepatic pathways and did not provoke any sign of toxic effects.
机译：辣根过氧化物酶（HRP）/ H 2 O 2介导的逆型乳液中多肽的交联是开发下一代生物相容性和可生物降解的纳米凝胶的有希望的方法。在此，我们提出了对三种表面活性剂的影响及其不同浓度对（HRP）/ H 2 O 2介导的纳米晶的纳米晶的基本面研究了聚[N5-（2-羟基乙基）-L-谷氨酰胺 - RAN-N5-丙基-1-谷氨酰胺 - Rann5-（6-氨基己基）-1-谷氨酰胺] - N5- [2-羟基苯基）乙基）-1-谷氨酸]（PHEG-TYR）逆型乳液。选择表面活性剂脱水山梨糖醇单烯醇（跨度80），聚氧乙基乙基三亚酸酯（TWEEN 85）和二辛基磺基琥珀酸钠钠盐（AOT），并评估对纳米凝胶尺寸，尺寸分布和形态的影响。用20wt％的非离子表面活性剂跨度80实现最有效的纳米蛋白稳定化。水凝胶纳米粒子的直径为230nm（动态光散射，DLS），并通过纳米颗粒跟踪分析（NTA）确认，显示直径范围为200到300 nm。显微镜和图像分析表明，干燥状态下的纳凝胶是球形的，并且具有数平均直径DN = 26nm和分散性。 = 1.91。在冷冻水合状态下，纳米凝胶出现多孔，尺寸较大，DN = 182nm，且＆ siC＆ = 1.52。我们的结果表明，聚合物前体的纳米凝胶化比经典反型乳液聚合更高浓度的表面活性剂，以确保有效稳定。将开发的多肽纳米凝胶用125I放射性标记，并进行体内生物分布和血液清除评估。我们发现，125个ilabeled纳米凝胶在血液中均匀突逐，通过肾和肝途径在48小时内从小鼠血液清除，并未引起任何毒性效果的迹象。

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  • 来源
    《Materials science & engineering》 |2021年第7期|111865.1-111865.9|共9页
  • 作者

    Oleshchuk Diana; Salek Petr; Dvorakova Jana; Kucka Jan; Pavlova Ewa; Francova Pavla; Sefc Ludek; Proks Vladimir;

  • 作者单位

    Czech Acad Sci Inst Macromol Chem Heyrovskeho Nam 2 Prague 16206 6 Czech Republic|Charles Univ Prague Dept Phys & Macromol Chem Fac Sci Hlavova 8 Prague 12800 2 Czech Republic;

    Czech Acad Sci Inst Macromol Chem Heyrovskeho Nam 2 Prague 16206 6 Czech Republic;

    Czech Acad Sci Inst Macromol Chem Heyrovskeho Nam 2 Prague 16206 6 Czech Republic;

    Czech Acad Sci Inst Macromol Chem Heyrovskeho Nam 2 Prague 16206 6 Czech Republic;

    Czech Acad Sci Inst Macromol Chem Heyrovskeho Nam 2 Prague 16206 6 Czech Republic;

    Charles Univ Prague Fac Med 1 Ctr Adv Preclin Imaging CAPI Salmovska 3 Prague 12000 2 Czech Republic;

    Charles Univ Prague Fac Med 1 Ctr Adv Preclin Imaging CAPI Salmovska 3 Prague 12000 2 Czech Republic;

    Czech Acad Sci Inst Macromol Chem Heyrovskeho Nam 2 Prague 16206 6 Czech Republic;

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  • 正文语种 eng
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  • 关键词

    Blood clearance; Inverse miniemulsion; in vivo biodistribution; Nanogel; Polypeptide; Surfactant;

    机译：血液清除;反向小乳液;体内生物分布;纳米凝胶;多肽;表面活性剂;

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