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首页> 外文期刊>Materials science & engineering >Development of a novel direct dental pulp-capping material using 4-META/MMA-TBB resin with nano hydroxyapatite
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Development of a novel direct dental pulp-capping material using 4-META/MMA-TBB resin with nano hydroxyapatite

机译：用纳米羟基磷灰石使用4-META / MMA-TBB树脂的新型直接牙髓覆盖材料的研制

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摘要

In the case of dental pulp exposure, direct pulp capping is often performed to preserve vital dental pulp tissue. Numerous studies regarding the development of direct pulp-capping materials have been conducted, but ma-terials with an appropriate sealing ability, which induce dense reparative dentin formation, have not been developed. Although nano hydroxyapatite (naHAp) is a bone-filling material with bioactivity and biocompati-bility, the inductive effects of naHAp on reparative dentin formation remain unclear. In the present study, the effects of dental adhesive material 4-methacryloxyethyl trimellitate anhydride/methyl methacrylate tri-n-butylborane [4-META/MMA-TBB or Super-bond (SB)], which included 10%, 30%, and 50% naHAp (naHAp/ SB) on odontoblastic differentiation of dental pulp stem cells (DPSCs) and reparative dentin formation were investigated. Scanning electron microscopy (SEM) and energy dispersive X-ray spectrometer analysis were performed to verify the existence of naHAp particles on the surface of naHAp/SB discs. The tensile adhesive strength of naHAp/SB was measured using a universal testing machine. As a result, 10% naHAp/SB and 30% naHAp/SB showed almost the same tensile adhesive strength as SB but 50% naHAp/SB showed significantly lower than the other experimental group. WST-1 proliferation assay and SEM analysis revealed that naHAp/SB did not affect the proliferation of DPSCs. Calcium release assay, quantitative RT-PCR, and western blotting analysis demonstrated that naHAp/SB did not release calcium ion but 30% naHAp/SB increased the expression of calcium-sensing receptor (CaSR) in DPSCs. Additionally, quantitative RT-PCR, western blotting analysis, Alizarin Red S-and von Kossa staining revealed that 30% naHAp/SB induced odontoblastic differentiation of DPSCs, which was inhibited by a MEK/ERK inhibitor and CaSR antagonist. Furthermore, 30% naHAp/SB promoted dense reparative dentin formation in an experimentally-formed rat dental pulp exposure model. These findings suggest that 30% naHAp/SB can be used as an ideal direct pulp capping material.
机译：在牙科纸浆暴露的情况下，通常进行直接纸浆覆盖物以保持生命的牙髓组织。已经进行了关于直接浆覆盖材料的发展的许多研究，但尚未开发出具有适当密封能力的MA-Terials，其诱导致密的牙本质形成。虽然纳米羟基磷灰石（NaHAP）是一种具有生物活性和生物相容性的骨填充材料，但是Nahap对Reparative Dentin形成的诱导效果仍不清楚。在本研究中，牙科粘合材料4-甲基丙烯酰氧基乙基三酸酐/甲基丙烯酸甲酯三-N-丁酯[4-META / MMA-TBB或超键（SB）]的影响，包括10％，30％和研究了对牙髓干细胞（DPSCs）和牙牙牙齿形成的异常细胞分化的50％NaHAP（NaHAP / Sb）。进行扫描电子显微镜（SEM）和能量分散X射线光谱仪分析以验证NaHAP / SB盘表面上的NaHAP颗粒的存在。使用通用试验机测量NaHAP / Sb的拉伸粘合强度。结果，10％NaHAP / Sb和30％NaHAP / Sb显示出几乎与Sb相同的拉伸粘合强度，但50％NaHAP / Sb显示显着低于其他实验组。 WST-1增殖测定和SEM分析显示，NaHAP / SB不影响DPSC的增殖。钙释放测定，定量RT-PCR和Western印迹分析表明，NaHAP / Sb未释放钙离子，但30％NaHAP / Sb增加了DPSC中钙传感受体（CasR）的表达。另外，定量RT-PCR，Western印迹分析，茜素红S和von Kossa染色显示，30％NaHAP / Sb诱导DPSC的异常分化，其被MEK / ERK抑制剂和CasR拮抗剂抑制。此外，在实验形成的大鼠牙科牙髓曝光模型中，30％NaHAP / SB促进了密集的牙本质形成。这些发现表明，30％NaHAP / Sb可用作理想的直接纸浆覆盖材料。

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  • 来源
    《Materials science & engineering》 |2021年第11期|112426.1-112426.14|共14页
  • 作者

    Yoshida Shinichiro; Sugii Hideki; Itoyama Tomohiro; Kadowaki Masataka; Hasegawa Daigaku; Tomokiyo Atsushi; Hamano Sayuri; Ipposhi Keita; Yamashita Kozue; Maeda Hidefumi;

  • 作者单位

    Kyushu Univ Hosp Div Endodontol Higashi Ku 3-1-1 Maidashi Fukuoka 8128582 Japan;

    Kyushu Univ Fac Dent Sci Dept Endodontol & Operat Dent Higashi Ku 3-1-1 Maidashi Fukuoka 8128582 Japan;

    Kyushu Univ Hosp Div Endodontol Higashi Ku 3-1-1 Maidashi Fukuoka 8128582 Japan;

    Kyushu Univ Fac Dent Sci Dept Endodontol & Operat Dent Higashi Ku 3-1-1 Maidashi Fukuoka 8128582 Japan;

    Kyushu Univ Fac Dent Sci Dept Endodontol & Operat Dent Higashi Ku 3-1-1 Maidashi Fukuoka 8128582 Japan;

    Kyushu Univ Hosp Div Endodontol Higashi Ku 3-1-1 Maidashi Fukuoka 8128582 Japan;

    Kyushu Univ Fac Dent Sci Dept Endodontol & Operat Dent Higashi Ku 3-1-1 Maidashi Fukuoka 8128582 Japan|Kyushu Univ Fac Dent Sci OBT Res Ctr Higashi Ku 3-1-1 Maidashi Fukuoka 8128582 Japan;

    Kyushu Univ Fac Dent Sci Dept Endodontol & Operat Dent Higashi Ku 3-1-1 Maidashi Fukuoka 8128582 Japan;

    Kyushu Univ Fac Dent Sci Dept Endodontol & Operat Dent Higashi Ku 3-1-1 Maidashi Fukuoka 8128582 Japan;

    Kyushu Univ Hosp Div Endodontol Higashi Ku 3-1-1 Maidashi Fukuoka 8128582 Japan|Kyushu Univ Fac Dent Sci Dept Endodontol & Operat Dent Higashi Ku 3-1-1 Maidashi Fukuoka 8128582 Japan;

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  • 正文语种 eng
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  • 关键词

    Dental pulp; Direct pulp capping; Nano hydroxyapatite; Reparative dentin; 4-META; MMA-TBB resin;

    机译：牙髓;直接纸浆封帽;纳米羟基磷灰石;重复牙本质;4-META;MMA-TBB树脂;

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