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首页> 外文期刊>Meat Science >Analysis of antibiotic resistance patterns and detection of mecA gene in Staphylococcus aureus isolated from packaged hamburger
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Analysis of antibiotic resistance patterns and detection of mecA gene in Staphylococcus aureus isolated from packaged hamburger

机译：包装汉堡包分离的金黄色葡萄球菌的抗生素耐药性模式分析及mecA基因检测

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摘要

Presence of Staphylococcus aureus, antibiotic resistance pattern and PCR detection of mecA gene in isolated strains were investigated in total of 256 packaged hamburgers in Iran-Tehran. For this purpose we used standard disk-diffusion method and sensitive and specific PCR technique, respectively. Results showed that 25% of samples were positive for S. aureus. Resistance to meticillin, erythromycin, penicillin G, cefazolin, ciprofloxasin, vacomy-cin and amoxiclave was determined 89%, 20.3%, 18.7%, 15.6%, 14%, 26.6% and 12.5%, respectively. According to the obtained results from PCR analysis of methicillin-resistant S. aureus (MRSA), mecA gene was present in 100% of the resistant isolates, 0% of intermediate-resistance isolates and 25% of susceptible isolates. The results obtained from PCR detection of mecA gene showed high correlation with standard disk diffusion test.
机译：在伊朗-德黑兰总共256个包装的汉堡包中调查了分离菌株中金黄色葡萄球菌的存在，抗生素耐药性模式和mecA基因的PCR检测。为此，我们分别使用了标准的磁盘扩散法和灵敏的特异性PCR技术。结果表明，有25％的样品对金黄色葡萄球菌呈阳性。确定对甲氧西林，红霉素，青霉素G，头孢唑林，环丙沙星，真空霉素和阿莫西韦的耐药性分别为89％，20.3％，18.7％，15.6％，14％，26.6％和12.5％。根据对耐甲氧西林的金黄色葡萄球菌（MRSA）进行PCR分析获得的结果，mecA基因存在于100％的抗性分离株，0％的中抗性分离株和25％的易感分离株中。通过PCR检测mecA基因获得的结果显示与标准盘扩散试验高度相关。

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  • 来源
    《Meat Science》 |2012年第3期|p.759-763|共5页
  • 作者

    Farzaneh Shahraz; Hasan Dadkhah; Ramin Khaksar; Maryam Mahmoudzadeh; Hedayat Hosseini; Manijeh Kamran; Paula Bourke;

  • 作者单位

    Department of Food Science and Technology, National Nutrition and Food Technology Research Institute. Faculty of Nutrition Science and Food Technology,Shaheed Beheshti University of Medical Sciences, Tehran, Iran;

    Department of Food Science and Technology, National Nutrition and Food Technology Research Institute. Faculty of Nutrition Science and Food Technology,Shaheed Beheshti University of Medical Sciences, Tehran, Iran;

    Department of Food Science and Technology, National Nutrition and Food Technology Research Institute. Faculty of Nutrition Science and Food Technology,Shaheed Beheshti University of Medical Sciences, Tehran, Iran;

    Department of Food Science and Technology, National Nutrition and Food Technology Research Institute. Faculty of Nutrition Science and Food Technology,Shaheed Beheshti University of Medical Sciences, Tehran, Iran;

    Department of Food Science and Technology, National Nutrition and Food Technology Research Institute. Faculty of Nutrition Science and Food Technology,Shaheed Beheshti University of Medical Sciences, Tehran, Iran;

    Department of Food Science and Technology, National Nutrition and Food Technology Research Institute. Faculty of Nutrition Science and Food Technology,Shaheed Beheshti University of Medical Sciences, Tehran, Iran;

    Food and Health Research Centre, School of Food Science and Environmental Health, Dublin Institute of Technology, Dublin, Ireland;

  • 收录信息 美国《科学引文索引》(SCI);
  • 原文格式 PDF
  • 正文语种 eng
  • 中图分类
  • 关键词

    MRSA; antibiotic resistance; staphylococcus aureus; hamburger; meca gene;

    机译：MRSA;抗生素耐药性;金黄色葡萄球菌;汉堡包;meca基因;

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