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首页> 外文期刊>Meat Science >New procedure for improving precision and accuracy of instrumental color measurements of beef
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New procedure for improving precision and accuracy of instrumental color measurements of beef

机译：提高牛肉仪器颜色测量精度和准确性的新程序

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摘要

The surface layers of steaks from bovine M. semimembranosus were prepared to have deoxy- (DMb), oxy- (OMb) and metmyoglobin (MMb) states using either chemicals (CHEM) or oxygen partial pressure packaging (OPP). Ninety-six different meat surface areas were measured in reflectance mode (400-1100 nm) for each preparation method. Reflectance spectra were converted to absorbance (A) and then transformed by Kubelka-Munk transformation (K/S) and/or extended multiplicative scatter correction (EMSC). Transformed spectra of prepared pure states were used to make calibration models of MMb, DMb and 0Mb using either selected wavelengths (SW) or partial least square (PLS) regression. Finally, the predicted myoglobin states were normalized to ensure that no state was <0 or >1 and the sum of all states equal to 1. Multivariate calibrations (i.e. PLS) outperformed the univariate calibrations (i.e. SW). The OPP method of preparing pure states was clearly best for 0Mb while the CHEM method was best for preparing MMb on fresh meat surfaces. Both preparation methods needed improvement concerning DMb. The CHEM(K/S) SW and the OPP EMSC(A) PLS methods predicted MMb, DMb and 0Mb with root-mean-square errors of cross validation (RMSECV) equal to 0.08,0.16 and 0.18 (range 0-1) and 0.04, 0.04 and 0.04 (range 0-1), respectively. This new reflectance protocol has potential for routine meat color measurements.
机译：使用化学药品（CHEM）或氧气分压包装（OPP），将牛半膜牛分枝杆菌的牛排表面层制备为具有脱氧（DMb），氧（OMb）和肌红蛋白（MMb）状态。对于每种制备方法，以反射模式（400-1100 nm）测量了九十六种不同的肉表面积。将反射光谱转换为吸光度（A），然后通过Kubelka-Munk转换（K / S）和/或扩展的倍增散射校正（EMSC）进行转换。使用选定的波长（SW）或偏最小二乘（PLS）回归，将准备好的纯态的变换光谱用于建立MMb，DMb和0Mb的校准模型。最后，对预测的肌红蛋白状态进行归一化以确保没有状态为<0或> 1，并且所有状态的总和等于1。多变量校准（即PLS）优于单变量校准（即SW）。显然，OPP制备纯态的方法最适合0Mb，而CHEM方法最适合制备新鲜肉类表面的MMb。两种制备方法都需要对DMb进行改进。 CHEM（K / S）SW和OPP EMSC（A）PLS方法预测MMb，DMb和0Mb的交叉验证的均方根误差（RMSECV）等于0.08、0.16和0.18（范围0-1），并且0.04、0.04和0.04（范围0-1）。这种新的反射协议具有常规肉色测量的潜力。

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  • 来源
    《Meat Science》 |2012年第3期|p.223-231|共9页
  • 作者

    Mamata Khatri; Vinh T. Phung; Tomas Isaksson; Oddvin Sørheim; Erik Slinde; Bjørg Egelandsdal;

  • 作者单位

    Department of Chemistry, Biotechnology and Food Science, University of Life Sciences, P. O. Box 5003, NO-1432 As, Norway;

    Department of Chemistry, Biotechnology and Food Science, University of Life Sciences, P. O. Box 5003, NO-1432 As, Norway;

    Department of Chemistry, Biotechnology and Food Science, University of Life Sciences, P. O. Box 5003, NO-1432 As, Norway;

    Nofima, Osloveien 1, NO-U30 As. Norway;

    Department of Chemistry, Biotechnology and Food Science, University of Life Sciences, P. O. Box 5003, NO-1432 As, Norway,Institute of Marine Research, P.O. Box 1870, Nordnes, NO-5817 Bergen, Norway;

    Department of Chemistry, Biotechnology and Food Science, University of Life Sciences, P. O. Box 5003, NO-1432 As, Norway;

  • 收录信息 美国《科学引文索引》(SCI);
  • 原文格式 PDF
  • 正文语种 eng
  • 中图分类
  • 关键词

    myoglobin state; reflectance; kubelka-munk transformation; partial least square regression; extended multiplicative scatter corrections;

    机译：肌红蛋白状态反射率kubelka-munk转换;偏最小二乘回归扩展的乘法散射校正;

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