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首页> 外文期刊>Meat Science >Determination of pig sex in meat and meat products using multiplex real time-PCR
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Determination of pig sex in meat and meat products using multiplex real time-PCR

机译：实时荧光定量PCR测定肉类和肉类产品中的猪性别

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摘要

For specific production lines, European retail companies demand exclusively female pork meat. To control the quality of their suppliers the identification and a quantitative detection of the animal sex origin of the meat is therefore of importance for meat processors. To enable a fast and reliable detection of male pig meat, a real time-PCR-system was designed in the present study. This was based on the genes AMEL-X and AMEL-Y. The real time-PCR assay allowed the detection of male pig meat at a concentration of 1% yielding a detection probability of 100% while the detection probability investigating meat samples containing 0.1% male pig meat was 44.4%. The analytic sensitivity of this system was assessed to be < 5 pg DNA per PCR reaction. The assessment of the accuracy of the real time-PCR assay to correctly identify sex individuals was investigated with 62 pigs including males (n = 29) and females (n = 33) belonging to different breeds/lines. With the newly designed test all analysed animals were correctly sexed. No amplification was obtained with cow, goat, sheep, turkey and chicken genomic DNA. The presented assay can be used for sex diagnosis, for the detection of male pig meat and for meat quality control.
机译：对于特定的生产线，欧洲零售公司仅要求女性猪肉。因此，为了控制供应商的质量，对肉类的动物性别起源进行识别和定量检测对于肉类加工者至关重要。为了能够快速可靠地检测雄性猪肉，本研究中设计了实时PCR系统。这是基于基因AMEL-X和AMEL-Y。实时PCR检测允许以1％的浓度检测雄性猪肉，检测概率为100％，而调查包含0.1％雄性猪肉的肉样品的检测概率为44.4％。该系统的分析灵敏度被评估为每个PCR反应<5 pg DNA。对62头猪进行了实时PCR检测以正确鉴定性别个体的准确性评估，其中包括不同品种/品系的雄性（n = 29）和雌性（n = 33）。通过新设计的测试，所有被分析的动物均已正确定性。牛，山羊，绵羊，火鸡和鸡的基因组DNA没有扩增。所提出的测定法可用于性别诊断，雄性猪肉的检测和肉质控制。

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  • 来源
    《Meat Science》 |2012年第3期|p.272-276|共5页
  • 作者

    A. Abdulmawjood; C. Krischek; M. Wicke; G. Klein;

  • 作者单位

    Institute of Food Quality and Food Safety, University of Veterinary Medicine, Hannover, Bischofsholer Damm 15, 30173 Hannover, Germany;

    Institute of Food Quality and Food Safety, University of Veterinary Medicine, Hannover, Bischofsholer Damm 15, 30173 Hannover, Germany;

    Department of Animal Sciences, Quality of Food of Animal Origin, Georg-August-University Coettingen, Albrecht-Thaer-Weg 3, d-37075 Goettingen, Germany;

    Institute of Food Quality and Food Safety, University of Veterinary Medicine, Hannover, Bischofsholer Damm 15, 30173 Hannover, Germany;

  • 收录信息 美国《科学引文索引》(SCI);
  • 原文格式 PDF
  • 正文语种 eng
  • 中图分类
  • 关键词

    pig; sexing; boar taint; AMEL; real time-PCR;

    机译：猪;性别公猪异味AMEL;实时PCR;

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