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首页> 外文期刊>Meat Science >Effect of solvent to sample ratio on total lipid extracted and fatty acid composition in meat products within different fat content
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Effect of solvent to sample ratio on total lipid extracted and fatty acid composition in meat products within different fat content

机译：不同脂肪含量下溶剂样品比对肉制品总脂质提取和脂肪酸组成的影响

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摘要

The effect of solvent to sample ratio on total extracted lipids and fatty acid (FA) composition in meat products with different fat contents was evaluated. Total lipids were extracted according to the Folch et al. (1957) method, using a 20:1 ratio of chloroform:methanol (2:1, v/v) to sample (A), and also testing the solvent:sample ratio of 10:1 (B). Higher amounts of total lipids and total FA from neutral lipids were obtained using the A ratio, which could be due to an insufficient chloroform:dry-weight sample proportion which could be insufficient for solubilizing the total amount of lipids. In the polar lipid fraction, the total amount of FA was higher using the B rather than the A ratio, which may be caused by the higher volume of added water when using A than B. When studying the FA composition of different lipid fractions, the volume of both the solvent and the water for total lipid extraction should be considered.
机译：评估了溶剂与样品的比例对不同脂肪含量的肉制品中总提取脂质和脂肪酸（FA）组成的影响。根据Folch等人的方法提取总脂质。 （1957）方法，使用氯仿：甲醇（2：1，v / v）与样品（A）的比例为20：1，并测试溶剂：样品的比例为（B）10：1。使用A比值可以从中性脂质中获得更高的总脂质和总FA含量，这可能是由于氯仿：干重样品比例不足，而该比例不足以溶解总脂质。在极性脂质组分中，使用B而不是A的比率，FA的总量更高，这可能是由于使用A时比B的加入水量更大。研究不同脂质组分的FA组成时，应考虑溶剂和水的总脂质提取量。

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  • 来源
    《Meat Science》 |2012年第3期|p.369-373|共5页
  • 作者

    T. Perez-Palacios; J. Ruiz; I.M.P.LV.O. Ferreira; C. Petisca; T. Antequera;

  • 作者单位

    REQUIMTE, Laboratorio de Bromatologia, Departamento de Quimica, Faculdade de Farmacia, Universidade do Porto, Rua Anibal Cunha 164,4099-030 Porto, Portugal,Tecnologia de los Alimentos, Facultad de Veterinaria, Universidad de Extremadura, Avda. de la Universidad s, 10071, Caceres, Spain;

    Tecnologia de los Alimentos, Facultad de Veterinaria, Universidad de Extremadura, Avda. De la Universidad s, 10071, Caceres, Spain;

    REQUIMTE, Laboratorio de Bromatologia, Departamento de Quimica, Faculdade de Farmacia, Universidade do Porto, Rua Anibal Cunha 164,4099-030 Porto, Portugal;

    REQUIMTE, Laboratorio de Bromatologia, Departamento de Quimica, Faculdade de Farmacia, Universidade do Porto, Rua Anibal Cunha 164,4099-030 Porto, Portugal;

    Tecnologia de los Alimentos, Facultad de Veterinaria, Universidad de Extremadura, Avda. De la Universidad s, 10071, Caceres, Spain;

  • 收录信息 美国《科学引文索引》(SCI);
  • 原文格式 PDF
  • 正文语种 eng
  • 中图分类
  • 关键词

    solvent:sample ratio; lipid extraction; neutral lipids; polar lipids; fatty acids;

    机译：溶剂：样品比例;脂质提取;中性脂质;极性脂质;脂肪酸;

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