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首页> 外文期刊>Meat Science >Analysis of pork adulteration in commercial meatballs targeting porcine-specific mitochondrial cytochrome b gene by TaqMan probe real-time polymerase chain reaction
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Analysis of pork adulteration in commercial meatballs targeting porcine-specific mitochondrial cytochrome b gene by TaqMan probe real-time polymerase chain reaction

机译:TaqMan探针实时聚合酶链反应分析靶向猪特异性线粒体细胞色素b基因的商品肉丸中的猪肉掺假

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摘要

A test for assessing pork adulteration in meatballs, using TaqMan probe real-time polymerase chain reaction, was developed. The assay combined porcine-specific primers and TaqMan probe for the detection of a 109 bp fragment of porcine cytochrome b gene. Specificity test with 10 ng DNA of eleven different species yielded a threshold cycle (Ct) of 15.5 ± 0.20 for the pork and negative results for the others. Analysis of beef meatballs with spiked pork showed the assay can determine 100-0.01% contaminated pork with 102% PCR efficiency, high linear regression (r~2 = 0.994) and <6% relative errors. Residuals analysis revealed a high precision in all determinations. Random analysis of commercial meatballs from pork, beef, chicken, mutton and goat, yielded a Ct between 15.89 ± 0.16 and 16.37 ± 0.22 from pork meatballs and negative results from the others, showing the suitability of the assay to determine pork in commercial meatballs with a high accuracy and precision.
机译:开发了一种使用TaqMan探针实时聚合酶链反应评估肉丸中猪肉掺假的测试。该检测方法结合了猪特异性引物和TaqMan探针,可检测到109 bp的猪细胞色素b基因片段。用11种不同物种的10 ng DNA进行的特异性测试得出的猪肉的阈值循环(Ct)为15.5±0.20,其他则为阴性。对加有猪肉的牛肉丸子的分析表明,该方法可以测定100-0.01%的污染猪肉,PCR效率为102%,线性回归较高(r〜2 = 0.994),相对误差<6%。残差分析显示所有测定均具有较高的精度。对猪肉,牛肉,鸡肉,羊肉和山羊肉的商品肉丸进行随机分析,得出猪肉肉丸的Ct在15.89±0.16和16.37±0.22之间,其他产品的阴性结果表明,该测定方法适用于测定具有以下特征的商品肉丸中的猪肉高精度和高精度。

著录项

  • 来源
    《Meat Science》 |2012年第4期|p.454-459|共6页
  • 作者单位

    Nanotechnology and Catalysis Research Center, Universiti Malaya. 50603 Kuala Lumpur, Malaysia;

    Institute ofNano Electronic Engineering, Universiti Malaysia Perils, 01000 Kangar, Perils, Malaysia;

    Halal Products Research Institute, Universiti Putra Malaysia, 43400 UPM Serdang, Selangor, Malaysia;

    Halal Products Research Institute, Universiti Putra Malaysia, 43400 UPM Serdang, Selangor, Malaysia;

    Institute ofNano Electronic Engineering, Universiti Malaysia Perils, 01000 Kangar, Perils, Malaysia;

    Institute ofNano Electronic Engineering, Universiti Malaysia Perils, 01000 Kangar, Perils, Malaysia;

    Institute of Tropical Agriculture, Universiti Putra Malaysia, 43400 UPM Serdang, Selangor, Malaysia;

    Nanotechnology and Catalysis Research Center, Universiti Malaya. 50603 Kuala Lumpur, Malaysia;

  • 收录信息 美国《科学引文索引》(SCI);
  • 原文格式 PDF
  • 正文语种 eng
  • 中图分类
  • 关键词

    meatball formulation; double quenched taqman probe; halal and kosher foods; threshold cycle; ground meat;

    机译:肉丸配方;双淬火taqman探针;清真和犹太食品;阈值周期;碎肉;

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