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首页> 外文期刊>IEEE / ASME Transactions on Mechatronics >ACAPELLA-1K: a biomechatronic fluid handling system for genome analysis that processes 1000 samples in 8 hours
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ACAPELLA-1K: a biomechatronic fluid handling system for genome analysis that processes 1000 samples in 8 hours

机译：ACAPELLA-1K：用于基因组分析的生物机电液处理系统，可在8小时内处理1000个样品

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摘要

An automated biomechatronic submicroliter fluid handling system for processing deoxyribonucleic acid (DNA) has been developed in the Genomation Laboratory, Department of Electrical Engineering, University of Washington, Seattle. This first generation system, ACAPELLA-1K, can process 1000 samples in 8 h in preparation for DNA sequencing using sample volumes ten times smaller than current state-of-the art manual and automated instrumentation. The system is based upon a proof-of-concept system that was developed by the Genomation Laboratory. The ACAPELLA-1K is the first integration of modules for fluid aspiration, dispensing, mixing, transport, and thermal processing that have been designed and developed with corporate partners Orca Photonic Systems, Inc., Redmond, WA, and Engineering Arts, Mercer Island, WA. These modules, comprising piezoceramic actuators, pneumatic pumps, linear mechanisms, thermal controllers, optical sensors, electronics, computer control, and software, are described in detail. Processing statistics are presented and successful experimental results are presented.
机译：西雅图华盛顿大学电气工程系Genomation实验室已开发出用于处理脱氧核糖核酸（DNA）的自动生物机电超微升流体处理系统。该第一代系统ACAPELLA-1K可以在8小时内处理1000个样品，从而为DNA测序做准备，所使用的样品量比当前最先进的手动和自动化仪器小十倍。该系统基于由Genomation实验室开发的概念验证系统。 ACAPELLA-1K是用于流体抽吸，分配，混合，运输和热处理的模块的首次集成，这些模块是与公司合作伙伴Orca Photonic Systems，Inc.（位于华盛顿州雷德蒙德）和Engineering Arts（位于默瑟岛，西澳。详细描述了这些模块，包括压电陶瓷执行器，气动泵，线性机构，热控制器，光学传感器，电子设备，计算机控制和软件。给出了处理统计数据，并给出了成功的实验结果。

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