...
  • 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Metabolomics >The influence of citrate, EDTA, and heparin anticoagulants to human plasma LC–MS lipidomic profiling
【24h】

The influence of citrate, EDTA, and heparin anticoagulants to human plasma LC–MS lipidomic profiling

机译：柠檬酸盐，EDTA和肝素抗凝剂对人血浆LC-MS脂质组分析的影响

获取原文
获取原文并翻译 | 示例
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

Lipid profiling of human plasma by liquid chromatography-electrospray ionization coupled to mass spectrometry (LC–ESI-MS) is being used to identify biomarkers of health, disease, and treatment efficacy. However, there is no consensus on the choice of anticoagulant to perform and compare lipidomic measurements. This study assessed the effect of the anticoagulants citrate, EDTA, and heparin, on eight synthetic and 80 plasma lipids, and compared lipidomic data among anticoagulants. Lipid extraction was affected distinctively by the anticoagulant of choice likely due to the different physico-chemical properties among anticoagulants. Peak areas of seventy endogenous lipids showed significant differences between citrate–heparin and EDTA–heparin comparisons similar to those observed for synthetic lipids. Only ten endogenous lipid species showed comparable peak areas among the three anticoagulants. Correction by a structurally related internal standard only partly eliminated differences among anticoagulants (ANOVA, P value <0.001). However, comparisons among anticoagulants were possible for most endogenous lipids after correction of peak areas by the sum of areas of its lipid class. Our observations indicate that the choice of anticoagulant distinctively impact the peak response of most lipid species by LC–ESI-MS. Lipidomic data from plasma obtained with different anticoagulants should address differences in matrix effects and extraction procedures since ion strength, plasma pH, and different physicochemical properties among anticoagulants influence lipid extraction and LC–ESI-MS analysis.
机译：通过液相色谱-电喷雾电离质谱联用技术（LC-ESI-MS）对人血浆进行脂质分析，可用于鉴定健康，疾病和治疗功效的生物标志物。然而，在选择抗凝剂进行和比较脂质组学测量方面尚无共识。这项研究评估了抗凝剂柠檬酸盐，EDTA和肝素对八种合成脂质和80种血浆脂质的作用，并比较了抗凝剂之间的脂质组学数据。由于抗凝剂之间的理化性质不同，因此选择的抗凝剂会显着影响脂质的提取。七十种内源性脂质的峰面积显示柠檬酸-肝素和EDTA-肝素的比较之间存在显着差异，与合成脂质相似。在三种抗凝剂中，只有十种内源脂质种类显示出可比的峰面积。通过与结构相关的内标进行校正只能部分消除抗凝剂之间的差异（ANOVA，P值<0.001）。然而，在通过其脂质类别的面积总和校正峰面积后，对于大多数内源性脂质，可以对抗凝剂进行比较。我们的观察表明，通过LC–ESI-MS，抗凝剂的选择会明显影响大多数脂质种类的峰响应。使用不同抗凝剂获得的血浆的脂血学数据应解决基质效应和萃取步骤的差异，因为抗凝剂之间的离子强度，血浆pH和不同的理化性质会影响脂质的萃取和LC-ESI-MS分析。

著录项

  • 来源
    《Metabolomics》 |2013年第2期|337-348|共12页
  • 作者

    Vanessa Gonzalez-Covarrubias; Adrie Dane; Thomas Hankemeier; Rob J. Vreeken;

  • 作者单位

    The Netherlands Metabolomics Centre">(1);

    LACDR Analytical Biosciences Leiden University">(2);

    The Netherlands Metabolomics Centre">(1);

    LACDR Analytical Biosciences Leiden University">(2);

    The Netherlands Metabolomics Centre">(1);

    LACDR Analytical Biosciences Leiden University">(2);

    The Netherlands Metabolomics Centre">(1);

    LACDR Analytical Biosciences Leiden University">(2);

  • 收录信息
  • 原文格式 PDF
  • 正文语种 eng
  • 中图分类
  • 关键词

    Anticoagulant; EDTA; Citrate; Heparin; Lipidomics; LC–MS;

    机译：抗凝物;EDTA;柠檬酸盐;肝素;脂质组学;液相色谱;

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号