Metabolic characterization of primary human colorectal cancers using high resolution magic angle spinning 1H magnetic resonance spectroscopy

M. Piotto; F.-M. Moussallieh; B. Dillmann; A. Imperiale; A. Neuville; C. Brigand; J.-P. Bellocq; K. Elbayed; I. J. Namer

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Metabolic characterization of primary human colorectal cancers using high resolution magic angle spinning 1H magnetic resonance spectroscopy

机译：高分辨率魔角旋转1 H磁共振波谱分析人类原发性大肠癌的代谢特征

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Colorectal cancer is one of the most frequent and most lethal forms of cancer in the western world. The aim of this study is to characterize by 1H high resolution magic angle spinning NMR spectroscopy (HRMAS) the metabolic fingerprint of both tumoral and healthy tissue samples obtained from a cohort of patients affected by primary colorectal adenocarcinoma. By analyzing HRMAS data using multivariate statistical analysis (PLS-DA), the two types of tissues could be discriminated with a high level of confidence. The identification of the metabolites at the origin of this discrimination revealed that adenocarcinomas are richer in taurine, glutamate, aspartate, and lactate whereas healthy tissues contain a higher amount of myo-inositol and β-glucose. The statistical model resulting from the PLS-DA analysis was subsequently used to perform a blind test on tumoral and healthy colon biopsies. The results of the classification showed that the HRMAS analysis has very high sensitivity and specificity.

机译：大肠癌是西方世界最常见，最致命的癌症之一。这项研究的目的是通过1 H高分辨率魔角旋转NMR光谱（HRMAS）表征从原发性大肠腺癌患者中获得的肿瘤和健康组织样品的代谢指纹。通过使用多元统计分析（PLS-DA）分析HRMAS数据，可以高置信度区分两种类型的组织。鉴定出这种区别的起源的代谢物表明，腺癌的牛磺酸，谷氨酸，天冬氨酸和乳酸含量更高，而健康组织中肌醇和β-葡萄糖的含量更高。随后将由PLS-DA分析得出的统计模型用于对肿瘤和健康的结肠活检进行盲测。分类结果表明，HRMAS分析具有很高的敏感性和特异性。

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《Metabolomics》 |2009年第3期|292-301|共10页
作者
M. Piotto; F.-M. Moussallieh; B. Dillmann; A. Imperiale; A. Neuville; C. Brigand; J.-P. Bellocq; K. Elbayed; I. J. Namer;
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作者单位

Bruker BioSpin 34 rue de l’industrie 67166 Wissembourg France;

Institut de Chimie Université Louis Pasteur 4 rue Blaise Pascal 67000 Strasbourg France;

Institut de Chimie Université Louis Pasteur 4 rue Blaise Pascal 67000 Strasbourg France;

Department of Biophysics and Nuclear Medicine University Hospitals of Strasbourg 67098 Strasbourg France;

Department of Pathology University Hospitals of Strasbourg 67098 Strasbourg France;

Department of Digestive Surgery University Hospitals of Strasbourg 67098 Strasbourg France;

Department of Pathology University Hospitals of Strasbourg 67098 Strasbourg France;

Institut de Chimie Université Louis Pasteur 4 rue Blaise Pascal 67000 Strasbourg France;

Department of Biophysics and Nuclear Medicine University Hospitals of Strasbourg 67098 Strasbourg France;

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正文语种 eng
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关键词
Colorectal cancer; Biopsies; HRMAS; Statistical analysis; Taurine; Myo-inositol;

机译：大肠癌;活检;HRMAS;统计分析;牛磺酸;肌醇;

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1. Metabolic Profiling of Human Colorectal Cancer Using High-Resolution Magic Angle Spinning Nuclear Magnetic Resonance (HR-MAS NMR) Spectroscopy and Gas Chromatography Mass Spectrometry (GC/MS) [J] . Chan ECY, Koh PK, Mal M, Journal of proteome research . 2009,第1期

机译：使用高分辨率魔角旋转核磁共振（HR-MAS NMR）光谱和气相色谱质谱（GC / MS）对人类大肠癌进行代谢谱分析
2. Metabolic characterization of distinct neuroanatomical regions in rats by magic angle spinning 1H nuclear magnetic resonance spectroscopy. [J] . Tsang TM, Griffin JL, Haselden J, Magnetic resonance in medicine: official journal of the Society of Magnetic Resonance in Medicine . 2005,第5期

机译：魔角旋转1H核磁共振波谱分析大鼠不同神经解剖区域的代谢特征。
3. Characterization of metabolic profile of intact non-tumor and tumor breast cells by high-resolution magic angle spinning nuclear magnetic resonance spectroscopy [J] . Maria Roberta M., Altei Wanessa F., Andricopulo Adriano D., Analytical Biochemistry: An International Journal of Analytical and Preparative Methods . 2015,第Null期

机译：高分辨率魔角旋转核磁共振波谱表征完整的非肿瘤和肿瘤乳腺细胞的代谢谱
4. Methyl Groups in Aquatic Humic Substances: Application of Ultra-Fast Magic Angle Spinning ~1H NMR Spectroscopy [C] . Ulrich Lankes, Fritz H. Frimmel the Meeting of the International Humic Substances Society . 2006

机译：水生腐烂物质中的甲基：超快魔法角度旋转〜1H NMR光谱的应用
5. High-resolution carbon-13 solid-state nuclear magnetic resonance: Decoupling and recoupling under high-speed magic angle spinning. [D] . Mehta, Anil Kumar. 1998

机译：高分辨率碳13固态核磁共振：高速魔角旋转下的去耦和再耦合。
6. Principal component analysis for the comparison of metabolic profiles from human rectal cancer biopsies and colorectal xenografts using high-resolution magic angle spinning 1H magnetic resonance spectroscopy [O] . Therese Seierstad, Kathrine Røe, Beathe Sitter, 2008

机译：主成分分析使用高分辨率魔角旋转1H磁共振光谱法比较人直肠癌活检组织和结直肠异种移植物的代谢谱
7. Principal component analysis for the comparison of metabolic profiles from human rectal cancer biopsies and colorectal xenografts using high-resolution magic angle spinning 1H magnetic resonance spectroscopy [O] . Seierstad, Therese, Røe, Kathrine, Sitter, Beathe, 2008

机译：主成分分析，使用高分辨率魔角旋转1H磁共振光谱法比较人直肠癌活检组织和结直肠异种移植物的代谢谱
8. High Resolution Magic Angle Spinning Nuclear Magnetic Resonance (HRMAS NMR) for Studies of Reactive Fabrics. [R] . McGarvey, D. J., Creasy, W. R. 2015

机译：用于反应性织物研究的高分辨率魔角旋转核磁共振（HRmas NmR）。

Metabolic characterization of primary human colorectal cancers using high resolution magic angle spinning 1H magnetic resonance spectroscopy

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