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Urine stability for metabolomic studies: effects of preparation and storage

机译：代谢组学研究的尿液稳定性：制备和储存的影响

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摘要

Metabolomic studies attempt to identify and profile unique metabolic differences among test populations, which may be correlated with a specific biological stress or pathophysiology. Due to the ease of collection and the metabolite-rich nature of urine, it is frequently used as a bio-fluid for human and animal metabolic studies. High-resolution 1H-NMR is an analytical tool used to qualitatively and quantitatively identify metabolites in urine. Urine samples were collected from healthy male and female subjects and prepared: raw, following centrifugation, filtration, or the addition of the bacteriostatic preservative sodium azide and analyzed by NMR. In addition, these samples were stored at room temperature (22 °C), in a refrigerator (4 °C), or in a deep-freeze (?80 °C). Samples were analyzed by NMR every week for a month and changes in concentrations of 55 easily identifiable metabolites were followed. The degree of change in metabolite concentrations following storage over a 4-week period were influenced by the different methods of sample preparation and storage. Significant changes in urine metabolites are likely due to bacterial contamination of the urine. Our study demonstrates that bacterial contamination of urine in normal individuals significantly alters the metabolic profile of urine over time and proper preparation and storage procedures must be followed to reduce these changes. By identifying appropriate methods of urine preparation and storage investigators will preserve the fidelity of the urine samples in order to better reflect the original metabolic state.
机译：代谢组学研究试图识别和描述测试人群之间独特的代谢差异，这可能与特定的生物应激或病理生理相关。由于尿液易于收集并且富含代谢产物，因此经常被用作人类和动物代谢研究的生物流体。高分辨率的1 H-NMR是用于定性和定量鉴定尿液中代谢物的分析工具。从健康的男性和女性受试者中收集尿液样品，并进行制备：粗制，离心，过滤或添加抑菌防腐剂叠氮化钠后，再进行NMR分析。另外，这些样品在室温（22°C），冰箱（4°C）或深度冷冻（？80°C）中存储。每周每周进行NMR分析样品，并追踪55种易于识别的代谢物的浓度变化。储存4周后代谢物浓度的变化程度受样品制备和储存方法不同的影响。尿液中的细菌污染可能会导致尿液代谢产物发生重大变化。我们的研究表明，正常个体尿液中的细菌污染会随着时间的推移显着改变尿液的代谢状况，因此必须遵循适当的准备和储存程序以减少这些变化。通过确定适当的尿液制备和储存方法，研究人员将保留尿液样品的保真度，以便更好地反映原始的代谢状态。

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  • 来源
    《Metabolomics》 |2007年第1期|19-27|共9页
  • 作者

    Erik J. Saude; Brian D. Sykes;

  • 作者单位

    CIHR Group in Protein Structure and Function University of Alberta Edmonton Alberta Canada;

    CIHR Group in Protein Structure and Function University of Alberta Edmonton Alberta Canada;

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  • 原文格式 PDF
  • 正文语种 eng
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  • 关键词

    metabolomic; urine; stability; bacteria; NMR; human;

    机译：代谢组学;尿;稳定性;细菌;NMR;人类;

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