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首页> 外文期刊>Microelectronic Engineering >Fabrication of 3D microdevices from planar electroplating for the isolation of cancer associated cells in blood
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Fabrication of 3D microdevices from planar electroplating for the isolation of cancer associated cells in blood

机译：从平面电镀制造3D微型电镀，用于血液中癌症相关细胞的分离

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摘要

Malignant cells detaching from a primary tumor to reach the bloodstream are known as circulating tumor cells (CTCs). Enumeration and molecular characterizations of these cells have been used as prognostic cancer biomarkers. Various in vitro techniques are available to extract these biomarkers from blood samples, although their performances are limited by the amount of blood available for analysis, usually 7.5 mL, and the rareness of these cells as a few per mL. To address this sampling bias we propose a three-dimensional (3D) microdevice adapted to the in vivo isolation of CTCs in the venous blood flow. Our device acts as an in vivo microfilter in which CTCs are specifically retained due to their specific morphological and mechanical properties as compared to normal blood cells. We present the fabrication process of these devices using planar silicon technology and their adaptation to generate a 3D microfilter integrated into a compatible medical consumable for venous access. We demonstrate that these devices are capable to capture human prostate cancer cells (PC3) spiked into whole blood using a fluidic platform mimicking an artificial vein. Moreover, the captured cells can be easily characterized by conventional microscopy and readily available for functional and downstream analysis. This minimally invasive technology could offer high-quality information to physicians and serve as a tool for personalized therapeutic follow-up in clinical routine.
机译：从原发性肿瘤脱离到血液的恶性细胞被称为循环肿瘤细胞（CTC）。这些细胞的枚举和分子表征已被用作预后癌生物标志物。可以使用各种体外技术从血液样品中提取这些生物标志物，尽管它们的性能受到可用于分析的血液量，通常为7.5ml，以及这些细胞的稀有量的每毫升的稀有量。为了解决这种采样偏压，我们提出了一种三维（3D）微小，适用于静脉血流中CTC的体内分离。我们的装置充当体内微型过滤器，其中与正常血细胞相比，由于它们的特定形态和机械性能，CTC被特异性保留。我们使用平面硅技术介绍了这些装置的制造过程及其适应，以产生集成的3D微型电机成兼容静脉通路的兼容医疗。我们证明这些装置能够使用模仿人造静脉的流体平台捕获尖刺到全血的人前列腺癌细胞（PC3）。此外，捕获的细胞可以通过常规显微镜容易地表征，并且容易可用于功能和下游分析。这种微创技术可以向医生提供高质量的信息，并作为临床常规的个性化治疗后续行动的工具。

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  • 来源
    《Microelectronic Engineering》 |2019年第5期|69-76|共8页
  • 作者

    Bou Elise; Jimenez-Zenteno Alejandro K.; Esteve Aurore; Bourrier David; Vieu Christophe; Cerf Aline;

  • 作者单位

    Univ Toulouse LAAS CNRS INSA UPS 7 Ave Colonel Roche F-31400 Toulouse France|SmartCatch 1 Ave Irene Joliot Curie F-31100 Toulouse France;

    Univ Toulouse LAAS CNRS INSA UPS 7 Ave Colonel Roche F-31400 Toulouse France;

    Univ Toulouse LAAS CNRS INSA UPS 7 Ave Colonel Roche F-31400 Toulouse France;

    Univ Toulouse LAAS CNRS INSA UPS 7 Ave Colonel Roche F-31400 Toulouse France;

    Univ Toulouse LAAS CNRS INSA UPS 7 Ave Colonel Roche F-31400 Toulouse France;

    Univ Toulouse LAAS CNRS INSA UPS 7 Ave Colonel Roche F-31400 Toulouse France;

  • 收录信息 美国《科学引文索引》(SCI);美国《工程索引》(EI);美国《生物学医学文摘》(MEDLINE);
  • 原文格式 PDF
  • 正文语种 eng
  • 中图分类
  • 关键词

    Circulating tumor cells; Microfiltration; Medical device; 3D fabrication; Electroplating;

    机译：循环肿瘤细胞;微滤;医疗器械;3D制造;电镀;

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