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Characterisation of the deflection of thin perforated SiN membranes of micro-hole array chips used for the cell selection

机译：用于细胞选择的微孔阵列芯片的多孔SiN薄膜的偏转特性

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摘要

An automated and reliable isolation of single cells is increasingly important for applications in the rapidly growing life science sector. Traditional methods, like limited dilution or cell-seeding in semi-solid media, are time-consuming, significantly limited by the number of clones that can be feasibly screened and not suitable for reliable generation of monoclonal cell lines. To enable a reliable isolation of single cell out of a high number of cells, a micro-hole array based fluidic system has been developed. Here single cells are positioned by using a suction pressure on the holes of a nitride membrane. The entrapped cells can be optically analysed and picked by a capillary system mounted on a micromanipulator. This picking process is working with small distances or contact between the capillary tip and the membrane of the micro-hole array chip. In this article the bending of the silicon nitride membrane is analysed in dependence of the applied suction pressure in order to know the resulting deflection offset (z-axis offset) at any position of the membrane. This enables a more reliable control of the picking procedure.
机译：对于迅速发展的生命科学领域中的应用，自动可靠地隔离单个细胞变得越来越重要。传统方法，如有限稀释或在半固体培养基中播种的细胞，非常耗时，受可筛选的克隆数量显着限制，不适合单克隆细胞系的可靠生成。为了能够可靠地从大量细胞中分离出单个细胞，已经开发了基于微孔阵列的流体系统。在此，通过利用抽吸压力将单个单元定位在氮化膜的孔上。可以通过安装在微操纵器上的毛细管系统对捕获的细胞进行光学分析和拾取。该拾取过程在毛细管尖端与微孔阵列芯片的膜之间的距离很小或接触很小的情况下起作用。在本文中，根据所施加的抽吸压力来分析氮化硅膜的弯曲，以便知道在膜的任何位置产生的偏转偏移（z轴偏移）。这使得可以更可靠地控制拣选过程。

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  • 来源
    《Microelectronic Engineering》 |2011年第8期|p.1782-1784|共3页
  • 作者

    Christian M. Kurz; Hagen Thielecke;

  • 作者单位

    Biohybrid Systems. Fraunhofer Institute for Biomedical Engineering. Ensheimer Str. 48, 66386 St. Ingbert, Germany;

    Biohybrid Systems. Fraunhofer Institute for Biomedical Engineering. Ensheimer Str. 48, 66386 St. Ingbert, Germany;

  • 收录信息 美国《科学引文索引》(SCI);美国《工程索引》(EI);美国《生物学医学文摘》(MEDLINE);
  • 原文格式 PDF
  • 正文语种 eng
  • 中图分类
  • 关键词

    cell micro-hole array; membrane deflection; cell picking;

    机译：细胞微孔阵列;膜偏转;细胞分选;

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