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首页> 外文期刊>Microgravity science and technology >Effects of Simulated Microgravity on Functions of Neutrophil-like HL-60 Cells
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Effects of Simulated Microgravity on Functions of Neutrophil-like HL-60 Cells

机译：模拟微重力对嗜中性粒细胞样HL-60细胞功能的影响

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摘要

Altered gravity, especially microgravity affects cellular functions of immune cells and can result in immune dysfunction for long-term, manned spaceflight and space exploration. The underlying mechanism, however, of sensing and responding to the gravity alteration is poorly understood. Here, a rotary cell culture system (RCCS) bioreactor was used to elucidate the effects of simulated microgravity on polymorphonuclear neutrophils (PMN)-like HL-60 cells. Alteration of cell morphology, up-regulation of (nitric oxide) NO production, enhancement of interleukin-6 (IL-6), interleukin-8 (IL-8), and monocyte chemotactic protein 1 (MCP-1) secretion, and diversity of cellular adhesion molecule expression were observed for the cells cultured in RCCS, leading to the up-regulated inflammatory immune responses and host defense. It was also indicated that such alterations in biological responses of PMNs mediated the reduced rolling velocity and decreased adhesion of PMN-like HL-60 cells on endothelial cells under shear flow. This work furthers the understandings in the effects and mechanism of microgravity on PMN functions, which are potentially helpful for optimizing the countermeasures to immune suppression in the future long-term, manned spaceflight.
机译：重力的改变，特别是微重力的改变会影响免疫细胞的细胞功能，并可能导致长期载人航天和太空探索的免疫功能障碍。然而，对重力变化的感知和响应的基本机制知之甚少。在这里，旋转细胞培养系统（RCCS）生物反应器用于阐明模拟微重力对多形核中性粒细胞（PMN）样HL-60细胞的影响。细胞形态的改变，（一氧化氮）NO产生的上调，白细胞介素6（IL-6），白细胞介素8（IL-8）和单核细胞趋化蛋白1（MCP-1）分泌的增加以及多样性在RCCS中培养的细胞中观察到细胞粘附分子表达的上调，导致炎症免疫反应和宿主防御的上调。还表明，在剪切流下，PMNs的生物学反应中的这种改变介导了滚动速度降低和PMN样HL-60细胞在内皮细胞上的粘附降低。这项工作进一步了解了微重力对PMN功能的影响和机理，这可能有助于优化未来长期载人航天飞行中的免疫抑制对策。

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  • 来源
    《Microgravity science and technology》 |2015年第6期|515-527|共13页
  • 作者

    Wang Chengzhi; Li Ning; Zhang Chen; Sun Shujin; Gao Yuxin; Long Mian;

  • 作者单位

    Chinese Acad Sci, Inst Mech, Ctr Biomech & Bioengn, Key Lab Micrograv,Natl Micrograv Lab, Beijing 100190, Peoples R China|Chinese Acad Sci, Inst Mech, Beijing Key Lab Engn Construct & Mechanobiol, Beijing 100190, Peoples R China;

    Chinese Acad Sci, Inst Mech, Ctr Biomech & Bioengn, Key Lab Micrograv,Natl Micrograv Lab, Beijing 100190, Peoples R China|Chinese Acad Sci, Inst Mech, Beijing Key Lab Engn Construct & Mechanobiol, Beijing 100190, Peoples R China;

    Chinese Acad Sci, Inst Mech, Ctr Biomech & Bioengn, Key Lab Micrograv,Natl Micrograv Lab, Beijing 100190, Peoples R China|Chinese Acad Sci, Inst Mech, Beijing Key Lab Engn Construct & Mechanobiol, Beijing 100190, Peoples R China;

    Chinese Acad Sci, Inst Mech, Ctr Biomech & Bioengn, Key Lab Micrograv,Natl Micrograv Lab, Beijing 100190, Peoples R China|Chinese Acad Sci, Inst Mech, Beijing Key Lab Engn Construct & Mechanobiol, Beijing 100190, Peoples R China;

    Chinese Acad Sci, Inst Mech, Ctr Biomech & Bioengn, Key Lab Micrograv,Natl Micrograv Lab, Beijing 100190, Peoples R China|Chinese Acad Sci, Inst Mech, Beijing Key Lab Engn Construct & Mechanobiol, Beijing 100190, Peoples R China;

    Chinese Acad Sci, Inst Mech, Ctr Biomech & Bioengn, Key Lab Micrograv,Natl Micrograv Lab, Beijing 100190, Peoples R China|Chinese Acad Sci, Inst Mech, Beijing Key Lab Engn Construct & Mechanobiol, Beijing 100190, Peoples R China;

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  • 原文格式 PDF
  • 正文语种 eng
  • 中图分类
  • 关键词

    HL-60; PMN; Effects of simulated microgravity; RCCS; Flow chamber;

    机译：HL-60;PMN;模拟微重力效应;RCCS;流室;

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