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首页> 外文期刊>Molecular Biology Reports >An easy method to detect the kinetics of CD44 antibody and its receptors on B16 cells using atomic force microscopy
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An easy method to detect the kinetics of CD44 antibody and its receptors on B16 cells using atomic force microscopy

机译：一种使用原子力显微镜检测B16细胞上CD44抗体及其受体动力学的简便方法

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CD44 is the principle cell surface receptor for the extracellular matrix. The altered expression or dysfunction of CD44 proteins contributes to numerous pathological processes. Therefore, it is very necessary to detect the distribution and density of CD44 proteins on cell surface. In this paper, the unbinding force between the tip of an atomic force microscope modified with anti-human CD44 antibody (a kind of CD44 pathway ligation proteins, currently used to induce the apoptosis of some types of tumors) and B16 (human melanoma cell line) cells was measured. The results indicated that the distribution of CD44 was nonuniform and represented clusters on B16 cell surface. And, the data of kinetics of CD44 antibody-antigen binding experiments indicated that the CD44 signal pathway in B16 cells could be blocked by anti-CD44 monoclonal antibody. This methodology can be extended to the evaluation and screening of molecular targeted drugs for pharmacological use.
机译：CD44是细胞外基质的主要细胞表面受体。 CD44蛋白的表达或功能异常会导致许多病理过程。因此，非常有必要检测细胞表面CD44蛋白的分布和密度。在本文中，用抗人CD44抗体（一种CD44途径连接蛋白，目前用于诱导某些类型的肿瘤细胞凋亡）修饰的原子力显微镜的尖端与B16（人黑素瘤细胞系）之间的解开力）进行了测量。结果表明，CD44的分布不均匀，代表B16细胞表面的簇。并且，CD44抗体-抗原结合实验的动力学数据表明，抗CD44单克隆抗体可以阻断B16细胞中的CD44信号通路。该方法可以扩展到用于药理学用途的分子靶向药物的评估和筛选。

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