• 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Molecular Biology Reports >A novel porcine gene, MAPKAPK3, is differentially expressed in the pituitary gland from mini-type Diannan small-ear pigs and large-type Diannan small-ear pigs
【24h】

A novel porcine gene, MAPKAPK3, is differentially expressed in the pituitary gland from mini-type Diannan small-ear pigs and large-type Diannan small-ear pigs

机译：新型滇南​​小耳猪和大型滇南小耳猪的垂体中有一个新的猪基因MAPKAPK3差异表达

获取原文
获取原文并翻译 | 示例
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

The mRNA differential display technique was performed to investigate the differences of gene expression in the pituitary gland from mini-type Diannan small-ear pigs and large-type Diannan small-ear pigs. One novel gene differentially expressed was identified through semi-quantitative RT-PCR and its cDNA complete sequence was then obtained using the rapid amplification of cDNA ends (RACE) method. Nucleotide sequence of the gene is not homologous to any of the known porcine genes. The sequence analysis revealed that the open reading frame of this gene encodes a protein of 384 amino acids has high homology with the mitogen-activated protein kinase-activated protein kinase 3 (MAPKAPK3) of eight species-cattle (96%), horse (96%), rhesus monkey (93%), rabbit (77%), human (98%), chimpanzee (98%), mouse (94%) and rat (91%)-so that it can be defined as swine MAPKAPK3 gene. This gene was finally assigned GENE ID: 100233192. Computer assisted analysis revealed that the genomic DNA of open reading frame of the swine MAPKAPK3 gene consists of ten exons and nine introns. The phylogenetic tree analysis revealed that the swine MAPKAPK3 gene has a closer genetic relationship with the MAPKAPK3 of cattle. The tissue expression analysis indicated that the MAPKAPK3 mRNA expression levels of large-type Diannan small-ear pigs are ubiquitously increased in various tissues compared to the mini-type Diannan small-ear pigs. Our experiment suggested that the swine MAPKAPK3 gene might play an important role in the growth and development differences between mini-type Diannan small-ear pigs and large-type Diannan small-ear pigs.
机译：进行了mRNA差异显示技术，以研究小型滇南小耳猪和大型滇南小耳猪的垂体基因表达差异。通过半定量RT-PCR鉴定了一个差异表达的新基因，然后使用cDNA末端快速扩增（RACE）方法获得了其cDNA完整序列。该基因的核苷酸序列与任何已知的猪基因都不同源。序列分析表明，该基因的开放阅读框编码一个384个氨基酸的蛋白质，与八种牛（96％），马（96％）的丝裂原活化蛋白激酶活化蛋白激酶3（MAPKAPK3）具有高度同源性。 ％），恒河猴（93％），兔（77％），人（98％），黑猩猩（98％），小鼠（94％）和大鼠（91％）-因此可以将其定义为猪MAPKAPK3基因。该基因最终被分配了GENE ID：100233192。计算机辅助分析表明，猪MAPKAPK3基因开放阅读框的基因组DNA由十个外显子和九个内含子组成。系统进化树分析表明，猪的MAPKAPK3基因与牛的MAPKAPK3有更密切的遗传关系。组织表达分析表明，与小型滇南小耳猪相比，大型滇南小耳猪的MAPKAPK3 mRNA表达水平普遍升高。我们的实验表明，猪MAPKAPK3基因可能在小型滇南小耳猪和大型滇南小耳猪之间的生长发育差异中起重要作用。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号