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首页> 外文期刊>Molecular BioSystems >Quantitative proteomic characterization of redox-dependent post-translational modifications on protein cysteines
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Quantitative proteomic characterization of redox-dependent post-translational modifications on protein cysteines

机译：蛋白质半胱氨酸氧化还原依赖性翻译后修饰的定量蛋白质组学表征

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摘要

Protein thiols play a crucial role in redox signaling, in the regulation of enzymatic activity and protein function, and in maintaining redox homeostasis in living systems. The unique chemical reactivity of the thiol group makes protein cysteines susceptible to reactions with reactive oxygen and nitrogen species that form various reversible and irreversible post-translational modifications (PTMs). The reversible PTMs in particular are major components of redox signaling and are involved in the regulation of various cellular processes under physiological and pathological conditions. The biological significance of these redox PTMs in both healthy and disease states has been increasingly recognized. Herein, we review recent advances in quantitative proteomic approaches for investigating redox PTMs in complex biological systems, including general considerations of sample processing, chemical or affinity enrichment strategies, and quantitative approaches. We also highlight a number of redox proteomic approaches that enable effective prof iling of redox PTMs for specific biological applications. Although technical limitations remain, redox proteomics is paving the way to a better understanding of redox signaling and regulation in both healthy and disease states.
机译：蛋白质硫醇在氧化还原信号传导，酶活性和蛋白质功能的调节以及维持生命系统氧化还原稳态方面起着至关重要的作用。巯基的独特化学反应性使半胱氨酸蛋白易于与活性氧和氮物种反应，形成各种可逆和不可逆的翻译后修饰（PTM）。可逆PTM特别是氧化还原信号的主要组成部分，并参与生理和病理条件下各种细胞过程的调节。这些氧化还原PTM在健康和疾病状态下的生物学意义已得到越来越多的认识。在本文中，我们回顾了定量蛋白质组学方法在研究复杂生物系统中氧化还原PTM方面的最新进展，包括样品处理，化学或亲和力富集策略以及定量方法的一般考虑。我们还将重点介绍许多氧化还原蛋白质组学方法，这些方法可以针对特定的生物学应用有效地生产氧化还原PTM。尽管仍然存在技术局限性，但是氧化还原蛋白质组学为更好地理解健康和疾病状态下的氧化还原信号传导和调节铺平了道路。

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  • 来源
    《Molecular BioSystems》 |2017年第5期|816-829|共14页
  • 作者

    Jicheng Duan; Matthew J. Gaffrey; Wei-Jun Qian;

  • 作者单位

    Integrative Omics Group, Biological Sciences Division, Pacific Northwest National Laboratory, Richland, WA 99352, USA.;

    Integrative Omics Group, Biological Sciences Division, Pacific Northwest National Laboratory, Richland, WA 99352, USA.;

    Integrative Omics Group, Biological Sciences Division, Pacific Northwest National Laboratory, Richland, WA 99352, USA.;

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