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首页> 外文期刊>Molecular Genetics and Genomics >Identification of microRNA-like RNAs in a plant pathogenic fungus Sclerotinia sclerotiorum by high-throughput sequencing
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Identification of microRNA-like RNAs in a plant pathogenic fungus Sclerotinia sclerotiorum by high-throughput sequencing

机译：高通量测序鉴定植物病原菌菌核盘菌中的microRNA样RNA

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摘要

MicroRNAs are small non-coding RNAs of approximately 22 nt with important regulatory roles in post-transcriptional regulation of gene expression. To date, no miRNAs have been reported in plant pathogenic fungi. Here we used Solexa sequencing to find new miRNAs in a plant pathogenic fungus Sclerotinia sclerotiorum. 13,229,401 high-quality reads were obtained, a total of 5,645,718 reads were mapped to the S. sclerotiorum genome corresponding to 348,108 unique sRNAs. Among the sRNAs, 2 microRNA-like RNAs (milRNAs) and 42 milRNA candidates were identified by sequence analysis. Northern blot and RT-PCR results showed that some milRNAs and candidates are differentially expressed in sclerotial development suggesting the role of these milRNAs and candidates in this biological process. Our results provide new insights into the functional roles of small RNAs and add new resources for the study of plant pathogenic fungi.
机译：MicroRNA是大约22 nt的小型非编码RNA，在基因表达的转录后调控中具有重要的调控作用。迄今为止，尚未在植物病原性真菌中报道miRNA。在这里，我们使用Solexa测序在植物致病真菌菌核盘菌中发现了新的miRNA。获得了13,229,401个高质量读段，总共5,645,718个读段被映射到了348.108个独特sRNA的核盘菌基因组中。在sRNA中，通过序列分析鉴定了2个类似microRNA的RNA（milRNA）和42个milRNA候选物。 Northern印迹和RT-PCR结果表明，一些milRNA和候选物在菌核发育中差异表达，表明这些milRNA和候选物在该生物学过程中的作用。我们的结果为小RNA的功能作用提供了新见解，并为植物病原性真菌的研究增加了新资源。

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