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Simultaneous occurrence of mycotoxins in human food commodities from Cameroon

机译:喀麦隆人类食品中同时发生霉菌毒素

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Eighty-two samples of dried food commodities from Cameroon were screened and quantified for different mycotoxins, including fumonisin B1 (FB1), zearalenone (ZEA), deoxynivalenol (DON), aflatoxin (AF) and ochratoxin A (OTA), by thin-layer chromatography (TLC) and high-performance liquid chromatography (HPLC), respectively. The percentage of positive samples was as follows: FB1 41%, AF 51%, ZEA 57%, DON 65% and OTA 3%. High FB1 contents were found in maize, averaging 3,684 µg/kg (range: 37-24,225 µg/kg), whereas the highest average ZEA level was found in peanuts (70 µg/kg), followed by maize (69 µg/kg), rice (67 µg/kg) and beans (48 µg/kg) with no ZEA was detected in soybeans. DON contents were low, ranging from 13 to 273 µg/kg, and for AF the average content was 2.6 µg/kg with peanuts and maize as principal substrates. The incidence of OTA was low, with a mean level of 6.4 µg/kg recorded. The majority (79%) of samples contained more than one mycotoxin and the most frequent co-occurrence found was FB1 + ZEA + DON, detected in 21% of samples (mainly maize) analysed. Co-contamination with FB1 + ZEA + DON + AF was found in 11% of the samples. Although a large proportion of samples had fairly low levels of individual mycotoxins, this should be of concern as the co-occurrence of mycotoxins may generate additive or synergistic effect in humans, especially if the respective commodities are consumed almost on a daily basis. Keywords Mycotoxins - Chromatography - Co-occurrence - Public health - Food commodities
机译:筛选并定量了来自喀麦隆的82份干食品的样品,以测定不同的真菌毒素,包括伏马毒素B 1 (FB 1 ),玉米赤霉烯酮(ZEA),脱氧雪腐酚(DON)分别通过薄层色谱法(TLC)和高效液相色谱法(HPLC)测定黄曲霉毒素(AF)和曲霉毒素A(OTA)。阳性样品的百分比如下:FB 1 41%,AF 51%,ZEA 57%,DON 65%和OTA 3%。玉米中的FB 1 含量高,平均为3,684 µg / kg(范围:37-24,225 µg / kg),而花生中的ZEA平均含量最高(70 µg / kg),其次在玉米中检测不到ZEA的玉米(69 µg / kg),大米(67 µg / kg)和豆类(48 µg / kg)。 DON含量低,在13至273 µg / kg范围内,而AF的平均含量为2.6 µg / kg,其中花生和玉米为主要基质。 OTA的发生率很低,记录的平均水平为6.4 µg / kg。大部分(79%)样品中含有一种以上霉菌毒素,发现的最常见的共生现象是FB 1 + ZEA + DON,在分析的21%样品(主要是玉米)中检出。在11%的样品中发现了与FB 1 + ZEA + DON + AF的共污染。尽管大部分样本中的个别霉菌毒素水平都相当低,但由于霉菌毒素的共同存在可能对人类产生加成或协同作用,因此应引起关注,特别是如果几乎每天都消费相应的商品。霉菌毒素-色谱-共现-公共卫生-食品

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