Monitoring of Ochratoxin A biosynthesis related genes in Penicillium verrucosum by differential display reverse transcription PCR (DDRT-PCR) experiments.

P. Färber; R. Geisen

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Monitoring of Ochratoxin A biosynthesis related genes in Penicillium verrucosum by differential display reverse transcription PCR (DDRT-PCR) experiments.

机译：通过差异显示逆转录PCR（DDRT-PCR）实验监测疣青霉中O曲霉毒素A生物合成相关基因。

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Differential display reverse transcription polymerase chain reaction experiments were performed to monitore Ochratoxin A biosynthetic genes inPenicillium verrucosum BFE487. A stringent prerequisite for the performance of this type of experiments is the knowledge of the differential expression of OTA genes. Differentially expressed total RNA from permissive/restrictive growth conditions and alternatively OTA+ wild type/OTA mutant strains have been isolated, reverse transcribed and used as a template in DDRT-PCR experiments. About 70 differentially expressed cDNA fragments could be isolated, cloned and sequenced.

机译：进行了差异显示逆转录聚合酶链反应实验，以监测青枯病菌BFE487中O曲霉毒素A的生物合成基因。进行这类实验的严格前提是必须了解OTA基因的差异表达。从容许/限制性生长条件和OTA +野生型/ OTA突变株中分离表达差异的总RNA，已分离，反转录并用作DDRT-PCR实验中的模板。可以分离，克隆和测序大约70个差异表达的cDNA片段。

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《Mycotoxin Research》 |2002年第s2期|124-127|共4页
作者
P. Färber; R. Geisen;
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Federal Research Center for Nutrition Institute of Hygiene;

Federal Research Center for Nutrition Institute of Hygiene;

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专利

1. Investigation of Ochratoxin A biosynthetic genes inPenicillium verrucosum by DDRT-PCR experiments: differential expression of OTA genes [J] . PF Färber, R Geisen Mycotoxin Research . 2001,第s2期

机译：DDRT-PCR实验研究疣白青霉中O曲霉毒素A生物合成基因：OTA基因的差异表达
2. IDENTIFICATION OF WOOD DECAY RELATED GENES FROM PIPTOPORUS BETULINUS (BULL. FR.) KARSTEN USING DIFFERENTIAL DISPLAY REVERSE TRANSCRIPTION PCR (DDRT-PCR) [J] . Meng Fanjuan, Liu Xin, Wang Qiuyun Biotechnology & Biotechnological Equipment . 2012,第3期

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3. Identification of Wood Decay Related Genes from Piptoporus Betulinus (Bull. Fr.) Karsten Using Differential Display Reverse Transcription PCR (DDRT-PCR) [J] . Fanjuan Meng, Qiuyun Wang, Xin Liu Biotechnology & Biotechnological Equipment . 2012,第3期

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4. FROM SINGLE SPORES TO MYCELIUM: VARIABILITY OF ASPERGILLUS WESTERDIJKIAE, A. CARBONARIUS AND PENICILLIUM VERRUCOSUM GROWTH AND OCHRATOXIN A PRODUCTION [C] . A.E. Kapetanakou, E. H. Drosinos, M. Mataragas, Πανελληνιο Συνεδριο ΤροΦιμων . 2012

机译：从单一孢子到菌丝体：产黄曲霉，碳弧菌和青霉菌的生长和AND曲霉毒素的变异性
5. Gene expression analysis of the proteinase inhibitor gene PiA of potato (Solanum tuberosum) by reverse transcription polymerase chain reaction (RT-PCR). [D] . Zhang, Tieling. 2003

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Monitoring of Ochratoxin A biosynthesis related genes in Penicillium verrucosum by differential display reverse transcription PCR (DDRT-PCR) experiments.

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