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首页> 外文期刊>NanoBioscience, IEEE Transactions on >Synthesis and Characterization of Double-layer Quantum-Dots-Tagged Microspheres
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Synthesis and Characterization of Double-layer Quantum-Dots-Tagged Microspheres

机译：双层量子点标记的微球的合成与表征

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Quantum-dots-tagged poly(styrene-acrylamide-acrylic acid) microspheres (QDsAAMs) were synthesized and modified with hydrazine hydrate through hydrazinolysis. Azidocarbonyl groups, which can be rapidly coupled with proteins under mild conditions, were introduced onto the surface of QDsAAM using azido reaction. Bovine serum albumin (BSA) was selected as model protein to be covalently immobilized on the azidocarbonyl QDsAAM. Instruments such as fluorescence microscope, optical microscope, confocal laser scanning microscope, UV-visible spectrometer, Fourier transform infrared spectrometer, size analyzer, and fluorescence spectrophotometer were used to characterize QDsAAM. Results showed that QDsAAM had a regular double-layer spherical shape and an average diameter of 11.2 mum. It also displayed high fluorescence intensity ( lambdaex/lambdaem=250 nm/370 nm), which showed linearity with concentrations ranging from 3.0times10-3 to 90.0times10-3gldrL-1. In addition, external factors such as pH and ionic strength exerted little influence on fluorescent characteristic. BSA immobilization indicated that QDsAAM with azidocarbonyl groups could be covalently coupled with BSA at the rate of 40times10-3 g/g (BSA/QDsAAM), while fluorescence linearity correlation was also found. This functional azidocarbonyl QDsAAM with sensitive fluorescence and active azidocarbonyl groups could be used as a promising fluorescent probe for quantitative detection, protein immobilization, and early rapid clinical diagnostics.
机译：合成了量子点标记的聚（苯乙烯-丙烯酰胺-丙烯酸）微球（QDsAAMs），并通过肼解作用用水合肼改性。使用叠氮基反应，可以在温和条件下与蛋白质快速偶联的叠氮基羰基被引入QDsAAM的表面。选择牛血清白蛋白（BSA）作为模型蛋白以共价固定在叠氮羰基QDsAAM上。使用荧光显微镜，光学显微镜，共聚焦激光扫描显微镜，紫外可见光谱仪，傅立叶变换红外光谱仪，尺寸分析仪和荧光分光光度计等仪器表征QDsAAM。结果表明，QDsAAM具有规则的双层球形，平均直径为11.2微米。它还显示出高荧光强度（lambdaex / lambdaem = 250 nm / 370 nm），其浓度在3.0×10-3至90.0×10-3gldrL-1范围内呈线性。另外，pH和离子强度等外部因素对荧光特性的影响很小。 BSA的固定化表明具有叠氮羰基的QDsAAM可以40×10-3 g / g（BSA / QDsAAM）与BSA共价偶联，同时还发现了荧光线性相关性。具有敏感荧光和活性叠氮羰基的功能性叠氮羰基QDsAAM可用作定量检测，蛋白质固定和早期快速临床诊断的有前途的荧光探针。

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