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首页> 外文期刊>NanoBioscience, IEEE Transactions on >Immune Response Induced by Fluorescent Nanocrystal Quantum Dots In Vitro and In Vivo
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Immune Response Induced by Fluorescent Nanocrystal Quantum Dots In Vitro and In Vivo

机译：荧光纳米晶体量子点诱导的免疫应答体内和体内

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Fluorescent nanocrystal quantum dots (QDs) are widely used as novel tools in various biological fields including cellular biology, molecular biology, and even in basic and clinical medical fields, due to their far brighter photoemission and photostability. Although many amounts of biological studies, including in vivo experiments, were circumstantially investigated, there is no informative report that investigates whether the QDs affect the mammalian immune system. This study investigated the immune response and biological behavior of QDs in vitro and in vivo. The immune response to QDs by both lymphocytes and kinds of macrophages in vitro and in vivo was investigated. Co-culture of QDs with immune cells showed that apparently normal production of cytokines and chemokines in both mouse CD4+ lymphocytes and peritoneal F4/80+ macrophages (PM phi). In addition, the bionanocomplex of QDs with enhanced-green-fluorescent-protein (eGFP)-encoding nucleotides successfully induced the expression of eGFP protein by PMphi. However, direct injection of QD+nucleotides bionanocomplex aqueous solution into the peritoneal cavity of mice resulted in the inflammation with the infiltration of inflammatory cells into the peritoneal cavity. Furthermore, QD+nucleotides bionanocomplex (but not QD bionanocomplex without nucleotides), induced the production of both proinflammatory cytokines and chemokines by PM phi in vitro. These results indicated that QDs covered with nucleotides caused the peritoneal inflammation in vivo via activation of PMphi and probably nonimmune cells. Taken together, these data indicated that QDs affect the proliferation of immune cells, but not in immune response including cytokine production. We propose here that all nanotechnology researchers should confirm the biological responses of their nanoscale products, because the biological response against nanoscale products can be occurred by not only in immune cells but also other nonimmune cells-n.
机译：荧光纳米晶体量子点（QDs）由于其更明亮的光发射和光稳定性而被广泛用作包括细胞生物学，分子生物学乃至基础和临床医学领域在内的各种生物学领域的新型工具。尽管已对包括体内实验在内的许多生物学研究进行了调查，但尚无有关QD是否影响哺乳动物免疫系统的信息性报告。这项研究调查了体外和体内量子点的免疫应答和生物学行为。研究了体内外淋巴细胞和巨噬细胞对QDs的免疫反应。 QD与免疫细胞的共培养表明，在小鼠CD4 +淋巴细胞和腹膜F4 / 80 +巨噬细胞（PM phi）中，细胞因子和趋化因子的产生均正常。此外，具有增强的绿色荧光蛋白（eGFP）编码核苷酸的QD的仿生复合物成功诱导了PMphi诱导eGFP蛋白的表达。但是，将QD +核苷酸仿生复合物水溶液直接注入小鼠腹膜腔会导致炎症，同时炎症细胞会渗入腹膜腔。此外，QD +核苷酸仿生复合物（但没有核苷酸的QD仿生复合物）则在体外诱导了phi phi促炎性细胞因子和趋化因子的产生。这些结果表明，被核苷酸覆盖的量子点通过激活PMphi和可能的非免疫细胞在体内引起腹膜炎症。总而言之，这些数据表明量子点影响免疫细胞的增殖，但不影响免疫应答，包括细胞因子的产生。我们在此建议所有纳米技术研究人员应确认其纳米级产物的生物学反应，因为针对纳米级产物的生物学反应不仅可以在免疫细胞中发生，而且还可以在其他非免疫细胞中发生。

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