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首页> 外文期刊>IEEE transactions on nanobioscience >Nano- and Microscale Holes Modulate Cell-Substrate Adhesion, Cytoskeletal Organization, and $-beta 1$ Integrin Localization in Sv40 Human Corneal Epithelial Cells
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Nano- and Microscale Holes Modulate Cell-Substrate Adhesion, Cytoskeletal Organization, and $-beta 1$ Integrin Localization in Sv40 Human Corneal Epithelial Cells

机译：纳米孔和微孔调节Sv40人角膜上皮细胞中的细胞基质粘附，细胞骨架组织和$ -beta 1 $整合素定位。

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摘要

Human corneal epithelial cells (HCECs) interface with a basement membrane in vivo that possesses complex nanoscale topographic features. We report that synthetic substrates patterned with nano- and microscale holes differentially modulate the proliferation, shape and adhesion of SV40 human corneal epithelial cells (SV40-HCECs) as a function of feature size: 1) Cell proliferation was inhibited on nanoscale features (features size less than 800 nm in pitch) compared to microscale features or planar substrates in identical culture conditions. 2)Cells on nanoscale holes had a stellate morphology compared to those on microscale features that were more evenly spread. 3) Cells adhered more to nanoscale features than to microscale features when exposed to shear stress in a laminar flow chamber. Transmission electron microscopy showed that cells cultured on the 400 nm pitch patterns had longer and more numerous filopodia and retraction fibers than cells cultured on the 1600 nm pitch patterns. Immunogold labeling of -beta1 integrins revealed that these receptors were localized at the cell periphery and in the aforementioned cytoskeletal elements. Our findings indicate that surface discontinuities and the activation of mechanochemical cell signaling mechanisms may contribute to the observed responses exhibited by SV40-HCECs cultured on nano- and microscale topography
机译：人角膜上皮细胞（HCEC）与体内具有复杂纳米尺度形貌特征的基底膜连接。我们报道，具有纳米和微米级孔的图案的合成基质差异地调节SV40人角膜上皮细胞（SV40-HCECs）的增殖，形状和粘附，其与特征尺寸有关：1）细胞增殖在纳米级特征（特征尺寸）上受到抑制与相同条件下的微尺度特征或平面基板相比，间距小于800 nm）。 2）与微观尺度上分布均匀的微孔相比，纳米尺度上的孔具有星状形态。 3）当在层流室内暴露于剪切应力时，细胞对纳米尺度特征的粘附要强于对微观尺度特征的粘附。透射电子显微镜显示，与以1600nm间距模式培养的细胞相比，以400nm间距模式培养的细胞具有更长和更多的丝状伪足和缩回纤维。 -β1整联蛋白的免疫金标记显示这些受体位于细胞外围和上述细胞骨架元件中。我们的发现表明，表面不连续性和机械化学细胞信号传导机制的激活可能有助于观察到的在纳米和微米级地形上培养的SV40-HCEC所显示的反应

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