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首页> 外文期刊>Nanobiotechnology, IET >Effect of green GO/Au nanocomposite on in-vitro amplification of human DNA
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Effect of green GO/Au nanocomposite on in-vitro amplification of human DNA

机译：绿色GO / Au纳米复合材料对人DNA体外扩增的影响

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摘要

Recently nanomaterials have attracted interest for increasing efficiency of polymerase chain reaction (PCR) systems. Here, the authors report on the usefulness of green graphene oxide/gold (GO/Au) nanocomposites for enhancement of PCR reactions. In this study, green GO/Au nanocomposite was prepared with Matricaria chamomilla extract as reducing/capping agent for site-directed nucleation of Au-o atoms on surface of GO sheets. The as-prepared green GO/Au nanocomposites were then characterised with UV-VIS spectrophotometer and scanning electron microscopy. Later, the effect of these nanocomposites was studied on end-point and real-time PCR employed for amplification of human glyceraldehyde-3-phosphate dehydrogenase gene. The results indicated that GO/Au nanocomposite can improve both end-point and real-time PCR methods at the optimum concentrations, possibly through interaction between GO/Au nanocomposite and the materials in PCR reaction, and through providing increased thermal convection by the GO surface as well as the Au nanostructures. In conclusion, it can be suggested that green GO/Au nanocomposite is a biocompatible and eco-friendly candidate as enhancer of in-vitro molecular amplification strategies.
机译：最近，纳米材料已引起人们对提高聚合酶链反应（PCR）系统效率的兴趣。在这里，作者报告了绿色氧化石墨烯/金（GO / Au）纳米复合材料对增强PCR反应的有用性。在这项研究中，制备了绿色GO / Au纳米复合材料，该材料以母菊提取物作为还原剂/封端剂，用于GO片表面Au-o原子的定点成核。然后用UV-VIS分光光度计和扫描电子显微镜表征所制备的绿色GO / Au纳米复合材料。后来，研究了这些纳米复合材料对终点和实时PCR扩增人类甘油醛-3-磷酸脱氢酶基因的影响。结果表明，GO / Au纳米复合材料可以在最佳浓度下改善终点和实时PCR方法，这可能是通过GO / Au纳米复合材料与PCR反应中的材料之间的相互作用，以及通过GO表面提供增加的热对流来实现的。以及金纳米结构。总之，可以认为绿色GO / Au纳米复合材料是生物相容性和生态友好的候选物，可以作为体外分子扩增策略的增强剂。

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