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首页> 外文期刊>Nanobiotechnology, IET >Effects of surface sterilisation with green synthesised silver nanoparticles on Lamiaceae seeds
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Effects of surface sterilisation with green synthesised silver nanoparticles on Lamiaceae seeds

机译：绿色合成的银纳米颗粒表面消毒对唇形科种子的影响

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摘要

Nanoparticles have been used in many areas of biotechnology. In this study, an alternative surface sterilisation method was established for plant tissue cultures. Silver nanoparticles synthesised via green synthesis were used for the surface sterilisation of Lamiaceae seeds (nSalvia farinecae, Ocimum basilicumn- Large Leaf Italian,nThymus vulgaris, Ocimum basilicumnvar.npurpurascens).nWater extracts of driednAlkanna tinctorumnrhizomes andnSyzygium aromaticumnflowers were utilised in the bioreduction of silver ions. The seeds were exposed to 0, 1, 7, 14 and 28 day-old colloidal solutions of silver nanoparticles and their effects on germination and surface sterilisation were determined. Fresh (0 and 1 day-old) colloidal solutions of silver nanoparticles were found very effective on surface sterilisation (100%). Moreover, they showed no negative effect on both germination and morphology of plantlets. It was shown that silver nanoparticles can be used as a surface sterilisation agent and they have no adverse effects on seed germination and in vitro plantlet growth.
机译：纳米颗粒已用于生物技术的许多领域。在这项研究中，建立了一种用于植物组织培养的替代性表面灭菌方法。通过绿色合成合成的银纳米颗粒用于唇形科种子的表面灭菌（n Salvia farinecae，罗勒属植物（Ocimum basilicum） n-大叶意大利语，n 百里香，罗勒属植物 nvar.n purpurascens）。 n干燥的水提取物 Alkanna tinctorum nrhizomes和n Syzygiumaromaticum nflowers被用于生物还原银离子。将种子暴露于0、1、7、14和28天的银纳米颗粒胶体溶液中，并测定其对发芽和表面灭菌的影响。发现银纳米颗粒的新鲜（0和1天大）胶体溶液对表面灭菌非常有效（100％）。而且，它们对苗的发芽和形态都没有负面影响。结果表明，银纳米颗粒可以用作表面杀菌剂，并且对种子发芽和离体苗生长没有不利影响。

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