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首页> 外文期刊>Nanobiotechnology, IET >Characterisation of optically driven microstructures for manipulating single DNA molecules under a fluorescence microscope
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Characterisation of optically driven microstructures for manipulating single DNA molecules under a fluorescence microscope

机译：荧光显微镜下用于操纵单个DNA分子的光学驱动微结构的表征

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摘要

Optical tweezers are powerful tools for manipulating single DNA molecules using fluorescence microscopy, particularly in nanotechnology-based DNA analysis. We previously proposed a manipulation technique using microstructures driven by optical tweezers that allows the handling of single giant DNA molecules of millimetre length that cannot be manipulated by conventional techniques. To further develop this technique, the authors characterised the microstructures quantitatively from the view point of fabrication and efficiency of DNA manipulation under a fluorescence microscope. The success rate and precision of the fabrications were evaluated. The results indicate that the microstructures are obtained in an aqueous solution with a precision ∼50 nm at concentrations in the order of 106 particles/ml. The visibility of these microstructures under a fluorescence microscope was also characterised, along with the elucidation of the fabrication parameters needed to fine tune visibility. Manipulating yeast chromosomal DNA molecules with the microstructures illustrated the relationship between the efficiency of manipulation and the geometrical shape of the microstructure. This report provides the guidelines for designing microstructures used in single DNA molecule analysis based on on-site DNA manipulation, and is expected to broaden the applications of this technique in the future.
机译：镊子是使用荧光显微镜操作单个DNA分子的强大工具，尤其是在基于纳米技术的DNA分析中。我们先前提出了一种使用由光镊驱动的微结构的操纵技术，该操纵技术允许处理常规技术无法操纵的毫米长的单个巨型DNA分子。为了进一步开发该技术，作者从荧光显微镜下DNA的制备和操作效率的角度定量地表征了微结构。评估了成功率和加工精度。结果表明，该微结构是在水溶液中以约106颗粒/ ml的浓度在约50 nm的精度下获得的。还表征了这些微结构在荧光显微镜下的可见性，以及阐明了微调可见性所需的制造参数。用微结构操纵酵母染色体DNA分子说明了操纵效率和微结构的几何形状之间的关系。该报告提供了基于现场DNA操作设计用于单个DNA分子分析的微结构的指南，并有望在将来扩展该技术的应用范围。

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