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首页> 外文期刊>Nanobiotechnology, IET >In situ detection of the Zn2+ release process of ZnO NPs in tumour cells by confocal laser scanning fluorescence microscopy
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In situ detection of the Zn2+ release process of ZnO NPs in tumour cells by confocal laser scanning fluorescence microscopy

机译：共聚焦激光扫描荧光显微镜原位检测肿瘤细胞中ZnO NPs Zn 2 + 的释放过程

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摘要

The use of zinc oxide (ZnO) nanoparticles (NPs) for cancer is not yet clear for human clinical applications, which is primarily due to the lack of a better understanding of the action mechanisms and cellular consequences of the direct exposure of cells to these NPs. In this work, the authors have selected zinquin ethyl ester, a Zn2+-specific fluorescent molecular probe, to efficiently differentiate ZnO NPs and Zn2+, and combined with confocal laser scanning microscopy (CLSM) to in situ study the Zn2+ release process of ZnO NPs in cancer cell system through detecting the change of Zn2+ level over time. During the experiments, the authors have designed the test group ZnO-2 in addition to assess the influence of a long-term storage on the characteristics of ZnO NPs in aqueous solution, and the Zn2+ release process of ZnO NPs in cancer cell system. After three-month storage at room temperature, the release process became earlier and faster, which was consistent with previous results of transmission electron microscope, UV-Vis and PL spectra. It is a good detection method that combination of Zn2+-specific fluorescent molecular probe and CLSM, which will be helpful for ZnO NPs using in clinical research.
机译：氧化锌（ZnO）纳米颗粒（NPs）在癌症上的用途在人类临床应用中尚不明确，这主要是由于对细胞直接暴露于这些NPs的作用机理和细胞后果缺乏更好的了解。在这项工作中，作者选择了Zn2 +特异性荧光分子探针锌喹乙酯来有效区分ZnO NP和Zn2 +，并与共聚焦激光扫描显微镜（CLSM）结合原位研究了ZnO NPs在ZnO中的释放过程。通过检测Zn2 +水平随时间的变化来检测癌细胞系统。在实验过程中，作者设计了ZnO-2测试组，以评估长期储存对水溶液中ZnO NPs特性的影响以及癌细胞系统中ZnO NPs的Zn2 +释放过程。在室温下保存三个月后，释放过程变得更快，更早，这与先前的透射电子显微镜，UV-Vis和PL光谱结果一致。 Zn2 +特异性荧光分子探针与CLSM的结合是一种很好的检测方法，对临床研究中的ZnO NPs有帮助。

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