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首页> 外文期刊>Nanobiotechnology, IET >Larvicidal activity of green synthesized silver nanoparticles using Excoecaria agallocha L. (Euphorbiaceae) leaf extract against Aedes aegypti
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Larvicidal activity of green synthesized silver nanoparticles using Excoecaria agallocha L. (Euphorbiaceae) leaf extract against Aedes aegypti

机译：使用Excoecaria agallocha L.（Euphorbiaceae）叶提取物的绿色合成银纳米颗粒对埃及伊蚊的杀幼虫活性

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摘要

Green synthesis of silver nanoparticles (AgNPs) using plant extracts has been achieved by eco-friendly reducing and capping agents. The present study was conducted to evaluate the larvicidal efficacies of AgNPs synthesized using aqueous leaf extracts of Excoecaria agallocha against dengue vector, Aedes aegypti. The 3rd and 4th instar larvae of A. aegypti were exposed to various concentrations of aqueous extracts of E. agallocha, synthesized AgNPs and also crude solvent extracts (methanol and chloroform) for 24 h. The formation of AgNPs using aqueous leaf extracts was observed after 30 min with a characteristic colour change. The results recorded from UV-Vis spectrum, XRD, FTIR, EDX, SEM and HR-TEM were used to characterize and confirm the biosynthesis of AgNPs. The highest larvicidal efficacy of synthesized AgNPs was observed against 3rd instar larvae at LC50 4.65 mg/L, LC90 14.17 mg/L and 4th instar larvae with a concentration of LC50 6.10 mg/L, LC90 15.64 mg/L. A significant larvicidal activity was also observed with crude methanolic extracts against 3rd instar larvae at a concentration LC50 41.74 mg/L, LC90 123.61 mg/L and 4th instar larvae at a concentration of LC50 52.06 mg/L, LC90 166.40 mg/L as compared to the chloroform extract.
机译：使用植物提取物通过生态友好型还原剂和加帽剂实现了绿色合成银纳米颗粒（AgNPs）。进行本研究以评估使用Excoecaria agallocha的叶片提取物对登革热媒介埃及伊蚊（Aedes aegypti）合成的AgNPs的杀幼虫功效。将埃及伊蚊的第3和第4龄幼虫暴露于各种浓度的水合提取物，合成的AgNPs以及粗溶剂提取物（甲醇和氯仿）中24小时。 30分钟后观察到使用水性叶提取物形成的AgNPs具有特征性的颜色变化。从紫外-可见光谱，XRD，FTIR，EDX，SEM和HR-TEM记录的结果用于表征和确认AgNPs的生物合成。观察到合成的AgNPs对3龄幼虫的最高杀幼虫功效为LC50 4.65 mg / L，LC90 14.17 mg / L和4龄幼虫，浓度为LC50 6.10 mg / L，LC90 15.64 mg / L。与之相比，粗甲醇提取物对浓度为LC50 41.74 mg / L，LC90 123.61 mg / L的三龄幼虫和浓度为LC50 52.06 mg / L，LC90 166.40 mg / L的四龄幼虫也具有明显的杀幼虫活性。氯仿提取物。

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