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首页> 外文期刊>Nanobiotechnology, IET >Electrospun cellulose acetate membrane for size separating and antibacterial screening of crude polysaccharides
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Electrospun cellulose acetate membrane for size separating and antibacterial screening of crude polysaccharides

机译：静电纺丝的醋酸纤维素膜，用于粗多糖的大小分离和抗菌筛选

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This study aims to produce electrospun cellulose acetate (CA) membrane as the alternative supporting medium for a separation of crude polysaccharides by electrophoresis and a screening of their antibacterial activity. Among the tested conditions of fabrication, electrospun CA membrane at 57% porosity showed the best separation of each polysaccharide from the standard mixture and the crude extract of Aloe vera via electrophoresis. As compared with the commercial CA membrane, the produced electrospun CA membrane demonstrated more separated spots of polysaccharides. The antibacterial activity of the electrophoretic polysaccharide was also determined against Escherichia coli and Staphylococcus aureus as the inhibition zone after the bacterial culture agar was overlaid on the membrane and incubated for 24 h. The results of this study suggested the potential application of electrospun CA membrane combining with electrophoresis as a simple method for separating crude polysaccharides and screening for their antibacterial activity.
机译：这项研究的目的是生产静电纺丝的醋酸纤维素（CA）膜，作为通过电泳分离粗多糖和筛选其抗菌活性的替代支持介质。在测试的制造条件中，电纺CA膜的孔隙率为57％，表明通过电泳从标准混合物和芦荟粗提物中分离出每种多糖的最佳效果。与商业CA膜相比，所生产的电纺CA膜表现出更多分离的多糖斑点。在将细菌培养琼脂覆盖在膜上并孵育24小时后，还测定了电泳多糖对大肠杆菌和金黄色葡萄球菌的抑菌活性，并将其作为抑制区域。这项研究的结果表明电纺CA膜结合电泳的潜在应用作为分离粗多糖并筛选其抗菌活性的简单方法。

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