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Colourimetric-based method for the diagnosis of spinal muscular atrophy using gold nanoprobes

机译：基于色度的金纳米探针诊断脊髓性肌萎缩的方法

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摘要

Although numerous molecular methods for spinal muscular atrophy (SMA) detection have been exploited, most of them are laborious, time consuming and costly. Recently, gold nanoparticles (AuNPs) have attracted attention in the field of colourimetric bioanalysis, because AuNP aggregation can be tracked with the naked eye as well as ultraviolet-visible (UV-vis) peak analysis. Here, based on a non-cross linking platform, a colourimetric-based method was used to evaluate the capability of thiolated oligo-AuNPs (Au nanoprobes) to distinguish between normal individuals, carriers and those with SMA. In this platform, removal of the repulsive force of the Au nanoprobes using high salt concentration solutions forced them to aggregate. Amplified DNA products from 20 blood samples were hybridised with the Au nanoprobes. UV-vis spectra and peak analysis ratios of SMA-positive samples revealed that, following salt addition, the unhybridised Au nanoprobes progressively aggregated and their absorption peak shifted to longer wavelengths ( <; 0.05), observed as a colour change from red to violet-purple. In contrast, colourimetric discrimination between normal and carrier samples following salt addition was not possible because of the small differences in their spectra and aggregation indices. Using this method, patients can be screened in <;30 min.
机译：尽管已经开发了许多用于检测脊髓肌萎缩症（SMA）的分子方法，但是大多数方法费力，费时且昂贵。最近，金纳米颗粒（AuNPs）在比色生物分析领域引起了人们的关注，因为可以用肉眼和紫外可见（UV-vis）峰分析来追踪AuNP聚集。在这里，基于非交联平台，基于比色法的方法用于评估硫醇化寡核苷酸-AuNP（Au纳米探针）区分正常个体，携带者和SMA患者的能力。在这个平台上，使用高盐浓度溶液去除Au纳米探针的排斥力迫使它们聚集。将来自20个血液样本的扩增DNA产物与Au纳米探针杂交。 SMA阳性样品的UV-vis光谱和峰分析比率表明，添加盐后，未杂交的Au纳米探针逐渐聚集，并且其吸收峰移至更长的波长（<; 0.05），观察到颜色从红色变为紫色。紫色。相反，添加盐后正常样品和载体样品之间的比色区分是不可能的，因为它们的光谱和聚集指数差异很小。使用此方法，可以在不到30分钟的时间内对患者进行筛查。

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