• 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Nanobiotechnology, IET >Electrospun scaffold containing TGF-β1 promotes human mesenchymal stem cell differentiation towards a nucleus pulposus-like phenotype under hypoxia
【24h】

Electrospun scaffold containing TGF-β1 promotes human mesenchymal stem cell differentiation towards a nucleus pulposus-like phenotype under hypoxia

机译：缺氧条件下含TGF-β1的电纺丝支架促进人间充质干细胞向髓核样表型的分化

获取原文
获取原文并翻译 | 示例
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

The study was aimed at evaluating the effect of electrospun scaffold containing TGF-β1 on promoting human mesenchymal stem cells (MSCs) differentiation towards a nucleus pulposus-like phenotype under hypoxia. Two kinds of nanofibrous scaffolds containing TGF-β1 were fabricated using uniaxial electrospinning (Group I) and coaxial electrospinning (Group II). Human MSCs were seeded on both kinds of scaffolds and cultured in a hypoxia chamber (2% O), and then the scaffolds were characterised. Cell proliferation and differentiation were also evaluated after 3 weeks of cell culture. Results showed that both kinds of scaffolds shared similar diameter distributions and protein release. However, Group I scaffolds were more hydrophilic than that of Group II. Both kinds of scaffolds induced the MSCs to differentiate towards the nucleus pulposus-type phenotype . In addition, the expression of nucleus pulposus-associated genes (aggrecan, type II collagen, HIF-1α and Sox-9) in Group I increased more than that of Group II. These results indicate that electrospinning nanofibrous scaffolds containing TGF-β1 supports the differentiation of MSCs towards the pulposus-like phenotype in a hypoxia chamber, which would be a more appropriate choice for nucleus pulposus regeneration.
机译：该研究旨在评估含TGF-β1的电纺丝支架在缺氧条件下促进人间充质干细胞（MSCs）向髓核样表型分化的作用。分别使用单轴电纺丝（I组）和同轴电纺丝（II组）制备了两种含TGF-β1的纳米纤维支架。将人MSCs接种在两种支架上，并在缺氧室（2％O）中培养，然后表征支架。细胞培养3周后，还评估了细胞增殖和分化。结果表明，两种支架都具有相似的直径分布和蛋白质释放。但是，第一组支架比第二组更亲水。两种支架均诱导MSC分化为髓核型表型。此外，第一组中髓核相关基因（aggrecan，II型胶原，HIF-1α和Sox-9）的表达比第二组增加。这些结果表明，含有TGF-β1的静电纺丝纳米纤维支架支持缺氧腔室中MSC向髓核样表型的分化，这将是髓核再生的更合适选择。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号