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首页> 外文期刊>Nanobiotechnology, IET >Phoenix dactylifera (date palm) pit aqueous extract mediated novel route for synthesis high stable silver nanoparticles with high antifungal and antibacterial activity
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Phoenix dactylifera (date palm) pit aqueous extract mediated novel route for synthesis high stable silver nanoparticles with high antifungal and antibacterial activity

机译： Phoenix dactylifera （枣棕）坑水提取物介导的合成高稳定性，具有高抗真菌和抗菌活性的银纳米颗粒的新途径

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摘要

The biological synthesis of silver nanoparticles (AgNPs) was conducted using date palm pit aqueous extract. The first visible sign of the synthesis of AgNPs was the change in colour of reaction mixtures from yellowish to reddish brown. The resulting synthesised AgNPs were characterised using UV-visible spectroscopy, X-ray diffraction (XRD), transmission electron microscopy (TEM) and dynamic light scattering (DLS). The UV-visible spectra gave surface plasmon resonance at 428 nm. XRD confirmed that the silver particles formed in our experiments were in the form of nanocrystals. TEM images revealed the formation of AgNPs with spherical shape and sizes in the range between 1–40 nm. DLS showed nanoparticles with an average size of 27 nm. Fourier transform infrared spectroscopy indicated the role of different possible functional groups (carboxyl, amine, aromatic and hydroxyl) in the formation of AgNPs. AgNPs were stable at 28°C for over a year without any precipitation or decreased production of antimicrobial effect. Then, the antifungal and antibacterial activities of synthesised AgNPs were investigated. The synthesised AgNPs showed significant inhibitory effects on (AG2_2) cultures, so that the concentration of 25 µg/ml prevented approximately 83% of the mycelium growth of the fungus. Then, the broth macro-dilution method was used for examining antibacterial effect of AgNPs. The minimum inhibitory concentration and minimum bactericide concentration against (PCI 602) and (ATCC 19606) were recorded as 1.56 and 3.12 µg/ml AgNPs, respectively.
机译：银纳米颗粒（AgNPs）的生物合成是使用枣棕坑水提取物进行的。 AgNPs合成的第一个可见标志是反应混合物的颜色从淡黄色变为红褐色。使用紫外可见光谱，X射线衍射（XRD），透射电子显微镜（TEM）和动态光散射（DLS）对得到的合成AgNP进行表征。紫外可见光谱在428 nm处产生表面等离子体共振。 XRD证实在我们的实验中形成的银颗粒是纳米晶体的形式。 TEM图像揭示了球形和尺寸在1–40 nm之间的AgNP的形成。 DLS显示纳米粒子的平均大小为27 nm。傅里叶变换红外光谱表明，AgNPs形成过程中可能具有不同的官能团（羧基，胺基，芳族和羟基）。 AgNP在28°C的温度下稳定一年以上，没有任何沉淀或抗菌作用降低。然后，研究了合成的AgNPs的抗真菌和抗菌活性。合成的AgNP对（AG2_2）培养物显示出显着的抑制作用，因此浓度为25 µg / ml可阻止约83％的真菌菌丝生长。然后，采用肉汤稀释法检测AgNPs的抗菌效果。对（PCI 602）和（ATCC 19606）的最小抑菌浓度和最小杀菌剂浓度分别记录为1.56和3.12 µg / ml AgNP。

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