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首页> 外文期刊>Naturwissenschaften >Export pumps in Epulopiscium fishelsoni, the symbiotic giant gut bacterium in Acanthurus nigrofuscus
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Export pumps in Epulopiscium fishelsoni, the symbiotic giant gut bacterium in Acanthurus nigrofuscus

机译：鱼Ac（Acanthurus nigrofuscus）中的共生大肠细菌共生鱼腥藻的出口泵

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摘要

Activity of the potential antixenobiotic efflux pumps of Epulopiscium fishelsoni (epulos), the symbiotic giant gut bacterium of the algivorous surgeonfish Acanthurus nigrofuscus, was studied in vivo using various specific substrates and microfluorometry. Kinetic and inhibitor analyses revealed the following vital efflux activities: (1) verapamil-sensitive efflux of amphiphilic cationic compounds rhodamine B, Hoechst 33342, and ethidium bromide; (2) verapamil-sensitive efflux of hydrophobic neutral fluo-rescein diacetate; (3) verapamil-insensitive efflux of hydro-philic anionic fluorescein; and (4) verapamil-insensitive efflux of glutathione-S-bimane. Cytosolic enzymes, nonspecific esterase and glutathione S-transferase, were shown to participate in xenobiotic metabolism. The results suggest that the activity of the potential efflux pump in epulos are similar to those described in other bacteria but are kinetically characterized by an unusually high transport rate, probably mediated by hyperplasia of the plasma membrane. Further studies of the export pumps in epulos may unmask their evolutionary adaptation to a xenobiotic-rich host gut content.
机译：使用各种特异性底物和微量荧光法，体内研究了食藻刺鱼棘形棘鱼的共生大肠细菌埃普罗球菌（epulos）的潜在异种外排泵的活性。动力学和抑制剂分析显示以下重要的外排活性：（1）两亲性阳离子化合物若丹明B，Hoechst 33342和溴化乙锭对维拉帕米敏感的外排； （2）疏水性中性氟树脂双乙酸盐对维拉帕米敏感的流出； （3）亲水性阴离子荧光素对维拉帕米不敏感的流出； （4）对谷胱甘肽-S-二mane对维拉帕米不敏感的外排。胞质酶，非特异性酯酶和谷胱甘肽S-转移酶被证明参与异种生物代谢。结果表明，潜在的外排泵的活动与其他细菌中所述的活动类似，但在动力学上具有异常高的传输速率，可能是由质膜的增生介导的。对埃普洛斯中出口泵的进一步研究可能揭示了它们对富含异种生物的宿主肠道含量的进化适应性。

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