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Carbonate - sensitive phytotransferrin controls high-affinity iron uptake in diatoms

机译：碳酸盐敏感的植物转铁蛋白可控制硅藻中高亲和力的铁吸收

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摘要

In vast areas of the ocean, the scarcity of iron controls the growth and productivity of phytoplankton(1,2). Although most dissolved iron in the marine environment is complexed with organic molecules(3), picomolar amounts of labile inorganic iron species (labile iron) are maintained within the euphotic zone(4) and serve as an important source of iron for eukaryotic phytoplankton and particularly for diatoms(5). Genome-enabled studies of labile iron utilization by diatoms have previously revealed novel iron responsive transcripts(6,7), including the ferric iron-concentrating protein ISIP2A(8), but the mechanism behind the acquisition of picomolar labile iron remains unknown. Here we show that ISIP2A is a phytotransferrin that independently and convergently evolved carbonate ion-coordinated ferric iron binding. Deletion of ISIP2A disrupts high-affinity iron uptake in the diatom Phaeodactylum tricornutum, and uptake is restored by complementation with human transferrin. ISIP2A is internalized by endocytosis, and manipulation of the seawater carbonic acid system reveals a second order dependence on the concentrations of labile iron and carbonate ions. In P. tricornutum, the synergistic interaction of labile iron and carbonate ions occurs at environmentally relevant concentrations, revealing that carbonate availability co-limits iron uptake. Phytotransferrin sequences have a broad taxonomic distribution(8) and are abundant in marine environmental genomic datasets(9,10), suggesting that acidification-driven declines in the concentration of seawater carbonate ions will have a negative effect on this globally important eukaryotic iron acquisition mechanism.
机译：在海洋的广大地区，铁的缺乏控制着浮游植物的生长和生产力（1,2）。尽管海洋环境中大多数溶解的铁都与有机分子复合（3），但在富营养区（4）内保持了皮摩尔量的不稳定无机铁物种（不稳定铁），并且是真核浮游植物特别是铁的重要来源对于硅藻（5）。基因组研究硅藻对不稳定铁的利用以前已经揭示了新颖的铁反应性转录物（6,7），包括浓缩三价铁的蛋白质ISIP2A（8），但是获得皮摩尔不稳定铁的背后机制仍然未知。在这里，我们显示ISIP2A是一种植物转铁蛋白，可独立且会聚地生成碳酸根离子配位的三价铁结合。 ISIP2A的缺失会破坏硅藻三角藻中高亲和力的铁吸收，并通过与人转铁蛋白互补来恢复吸收。 ISIP2A通过内吞作用而被内在化，对海水碳酸系统的操纵揭示了其对不稳定铁和碳酸根离子浓度的二阶依赖性。在P. tricornutum中，不稳定的铁和碳酸盐离子的协同相互作用发生在与环境相关的浓度下，这表明碳酸盐可利用性共同限制了铁的吸收。植物转铁蛋白序列具有广泛的生物分类分布（8），并且在海洋环境基因组数据集中具有丰富的生物分类（9，10），这表明酸化驱动的海水碳酸盐离子浓度下降将对该全球重要的真核生物铁捕获机制产生负面影响。 。

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  • 来源
    《Nature》 |2018年第7697期|534-537|共4页
  • 作者

    MeQuaid Jeffrey B.; Kustka Adam B.; Obornik Miroslav; Horak Ales; McCrow John R.; Karas Bogumil J.; Zheng Hong; Kindeberg Theodor; Andersson Andreas J.; Barbeau Katherine A.; Allen Andrew E.;

  • 作者单位

    J Craig Venter Inst, Microbial & Environm Genom, La Jolla, CA 92037 USA;

    Rutgers State Univ, Earth & Environm Sci, Newark, NJ 07102 USA;

    Inst Parasitol, Biol Ctr CAS, Branisovska 31, Ceske Budejovice 37005, Czech Republic;

    Inst Parasitol, Biol Ctr CAS, Branisovska 31, Ceske Budejovice 37005, Czech Republic;

    J Craig Venter Inst, Microbial & Environm Genom, La Jolla, CA 92037 USA;

    J Craig Venter Inst, Microbial & Environm Genom, La Jolla, CA 92037 USA;

    J Craig Venter Inst, Microbial & Environm Genom, La Jolla, CA 92037 USA;

    Univ Calif La Jolla, Scripps Inst Oceanog, La Jolla, CA 92093 USA;

    Univ Calif La Jolla, Scripps Inst Oceanog, La Jolla, CA 92093 USA;

    Univ Calif La Jolla, Scripps Inst Oceanog, La Jolla, CA 92093 USA;

    J Craig Venter Inst, Microbial & Environm Genom, La Jolla, CA 92037 USA;

  • 收录信息 美国《科学引文索引》(SCI);美国《工程索引》(EI);美国《生物学医学文摘》(MEDLINE);美国《化学文摘》(CA);
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