Integrative structure and functional anatomy of a nuclear pore complex

Kim Seung Joong; Fernandez-Martinez Javier; Nudelman Ilona; Shi Yi; Zhang Wenzhu; Raveh Barak; Herricks Thurston; Slaughter Brian D.; Hogan Joanna A.; Upla Paula; Chemmama Ilan E.; Pellarin Riccardo; Echeverria Ignacia; Shivaraju Manjunatha; Chaudhury Azraa S.; Wang Junjie; Williams Rosemary; Unruh Jay R.; Greenberg Charles H.; Jacobs Erica Y.; Yu Zhiheng; de la Cruz M. Jason; Mironska Roxana; Stokes David L.; Aitchison John D.; Jarrold Martin F.; Gerton Jennifer L.; Ludtke Steven J.; Akey Christopher W.; Chait Brian T.; Sali Andrej; Rout Michael P.

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Integrative structure and functional anatomy of a nuclear pore complex

机译：核孔复合体的整体结构和功能解剖

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Nuclear pore complexes play central roles as gatekeepers of RNA and protein transport between the cytoplasm and nucleoplasm. However, their large size and dynamic nature have impeded a full structural and functional elucidation. Here we determined the structure of the entire 552-protein nuclear pore complex of the yeast Saccharomyces cerevisiae at sub-nanometre precision by satisfying a wide range of data relating to the molecular arrangement of its constituents. The nuclear pore complex incorporates sturdy diagonal columns and connector cables attached to these columns, imbuing the structure with strength and flexibility. These cables also tie together all other elements of the nuclear pore complex, including membrane-interacting regions, outer rings and RNA-processing platforms. Inwardly directed anchors create a high density of transport factor-docking Phe-Gly repeats in the central channel, organized into distinct functional units. This integrative structure enables us to rationalize the architecture, transport mechanism and evolutionary origins of the nuclear pore complex.

机译：核孔复合体起着RNA和蛋白质在细胞质和核质之间转运的守门员的核心作用。但是，它们的大尺寸和动态性质阻碍了对结构和功能的充分阐明。在这里，我们通过满足有关其成分的分子排列的广泛数据，确定了亚酿酒酵母的整个552蛋白核孔复合物的结构。核孔复合体包含坚固的对角柱和连接到这些柱的连接器电缆，使结构具有强度和灵活性。这些电缆还将核孔复合体的所有其他元素紧密结合在一起，包括膜相互作用区域，外环和RNA处理平台。向内定向的锚在中央通道中形成高密度的转运因子对接Phe-Gly重复序列，组织成不同的功能单元。这种整合结构使我们能够合理化核孔复合体的结构，转运机制和进化起源。

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《Nature》 |2018年第7697期|475-482|共8页
作者
Kim Seung Joong; Fernandez-Martinez Javier; Nudelman Ilona; Shi Yi; Zhang Wenzhu; Raveh Barak; Herricks Thurston; Slaughter Brian D.; Hogan Joanna A.; Upla Paula; Chemmama Ilan E.; Pellarin Riccardo; Echeverria Ignacia; Shivaraju Manjunatha; Chaudhury Azraa S.; Wang Junjie; Williams Rosemary; Unruh Jay R.; Greenberg Charles H.; Jacobs Erica Y.; Yu Zhiheng; de la Cruz M. Jason; Mironska Roxana; Stokes David L.; Aitchison John D.; Jarrold Martin F.; Gerton Jennifer L.; Ludtke Steven J.; Akey Christopher W.; Chait Brian T.; Sali Andrej; Rout Michael P.;
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作者单位

Univ Calif San Francisco, Dept Pharmaceut Chem, Dept Bioengn & Therapeut Sci, San Francisco, CA 94158 USA;

Rockefeller Univ, Lab Cellular & Struct Biol, New York, NY 10065 USA;

Rockefeller Univ, Lab Cellular & Struct Biol, New York, NY 10065 USA;

Rockefeller Univ, Lab Mass Spectrometry & Gaseous Ion Chem, New York, NY 10065 USA;

Rockefeller Univ, Lab Mass Spectrometry & Gaseous Ion Chem, New York, NY 10065 USA;

Univ Calif San Francisco, Dept Pharmaceut Chem, Dept Bioengn & Therapeut Sci, San Francisco, CA 94158 USA;

Inst Syst Biol, 401 Terry Ave N, Washington, DC 93109 USA;

Stowers Inst Med Res, Kansas City, MO 64110 USA;

Indiana Univ, Dept Chem, Bloomington, IN 47405 USA;

NYU, Sch Med, Skirball Inst, New York, NY 10016 USA;

Univ Calif San Francisco, Dept Pharmaceut Chem, Dept Bioengn & Therapeut Sci, San Francisco, CA 94158 USA;

Univ Calif San Francisco, Dept Pharmaceut Chem, Dept Bioengn & Therapeut Sci, San Francisco, CA 94158 USA;

Univ Calif San Francisco, Dept Pharmaceut Chem, Dept Bioengn & Therapeut Sci, San Francisco, CA 94158 USA;

Stowers Inst Med Res, Kansas City, MO 64110 USA;

Rockefeller Univ, Lab Cellular & Struct Biol, New York, NY 10065 USA;

Rockefeller Univ, Lab Mass Spectrometry & Gaseous Ion Chem, New York, NY 10065 USA;

Rockefeller Univ, Lab Cellular & Struct Biol, New York, NY 10065 USA;

Stowers Inst Med Res, Kansas City, MO 64110 USA;

Univ Calif San Francisco, Dept Pharmaceut Chem, Dept Bioengn & Therapeut Sci, San Francisco, CA 94158 USA;

Rockefeller Univ, Lab Mass Spectrometry & Gaseous Ion Chem, New York, NY 10065 USA;

Janelia Res Campus, Howard Hughes Med Inst, 19700 Helix Dr, Ashburn, VA 20148 USA;

Janelia Res Campus, Howard Hughes Med Inst, 19700 Helix Dr, Ashburn, VA 20148 USA;

Rockefeller Univ, Lab Cellular & Struct Biol, New York, NY 10065 USA;

NYU, Sch Med, Skirball Inst, New York, NY 10016 USA;

Ctr Infect Dis Res, Seattle, WA 98109 USA;

Indiana Univ, Dept Chem, Bloomington, IN 47405 USA;

Stowers Inst Med Res, Kansas City, MO 64110 USA;

Baylor Coll Med, Verna & Marrs McLean Dept Biochem & Mol Biol, 1 Baylor Plaza, Houston, TX 77030 USA;

Boston Univ, Sch Med, Dept Physiol & Biophys, 700 Albany St, Boston, MA 02118 USA;

Rockefeller Univ, Lab Mass Spectrometry & Gaseous Ion Chem, New York, NY 10065 USA;

Univ Calif San Francisco, Dept Pharmaceut Chem, Dept Bioengn & Therapeut Sci, San Francisco, CA 94158 USA;

Rockefeller Univ, Lab Cellular & Struct Biol, New York, NY 10065 USA;

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收录信息美国《科学引文索引》(SCI);美国《工程索引》(EI);美国《生物学医学文摘》(MEDLINE);美国《化学文摘》(CA);
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机译：核心络合物的一体化结构和功能解剖学

Integrative structure and functional anatomy of a nuclear pore complex

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