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首页> 外文期刊>Nature >A flavin-based extracellular electron transfer mechanism in diverse Gram-positive bacteria
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A flavin-based extracellular electron transfer mechanism in diverse Gram-positive bacteria

机译：基于黄素的多种革兰氏阳性细菌中的细胞外电子转移机制

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摘要

Extracellular electron transfer (EET) describes microbial bioelectrochemical processes in which electrons are transferred from the cytosol to the exterior of the cell(1). Mineral-respiring bacteria use elaborate haem-based electron transfer mechanisms(2-4) but the existence and mechanistic basis of other EETs remain largely unknown. Here we show that the food-borne pathogen Listeria monocytogenes uses a distinctive flavin-based EET mechanism to deliver electrons to iron or an electrode. By performing a forward genetic screen to identify L. monocytogenes mutants with diminished extracellular ferric iron reductase activity, we identified an eight-gene locus that is responsible for EET. This locus encodes a specialized NADH dehydrogenase that segregates EET from aerobic respiration by channelling electrons to a discrete membrane-localized quinone pool. Other proteins facilitate the assembly of an abundant extracellular flavoprotein that, in conjunction with free-molecule flavin shuttles, mediates electron transfer to extracellular acceptors. This system thus establishes a simple electron conduit that is compatible with the single-membrane structure of the Gram-positive cell. Activation of EET supports growth on non-fermentable carbon sources, and an EET mutant exhibited a competitive defect within the mouse gastrointestinal tract. Orthologues of the genes responsible for EET are present in hundreds of species across the Firmicutes phylum, including multiple pathogens and commensal members of the intestinal microbiota, and correlate with EET activity in assayed strains. These findings suggest a greater prevalence of EET-based growth capabilities and establish a previously underappreciated relevance for electrogenic bacteria across diverse environments, including host-associated microbial communities and infectious disease.
机译：细胞外电子转移（EET）描述了微生物生物电化学过程，其中电子从胞质溶胶转移到细胞外部（1）。矿物质呼吸细菌使用复杂的基于血红素的电子转移机制（2-4），但其他EET的存在和机制基础仍然未知。在这里，我们表明食源性单核细胞增生李斯特氏菌利用一种基于黄素的独特EET机制将电子传递至铁或电极。通过执行向前的遗传筛选，以识别具有减少的胞外三价铁还原酶活性的单核细胞增生李斯特氏菌突变体，我们确定了一个负责EET的八基因位点。该基因座编码一种专门的NADH脱氢酶，该酶通过将电子引导至离散的膜局部醌池将EET与有氧呼吸隔离。其他蛋白质促进了丰富的细胞外黄素蛋白的组装，与自由分子黄素穿梭蛋白结合，介导电子转移至细胞外受体。因此，该系统建立了与革兰氏阳性细胞的单膜结构兼容的简单电子导管。 EET的激活支持非发酵碳源上的生长，并且EET突变体在小鼠胃肠道内表现出竞争性缺陷。负责EET的基因的直向同源物存在于整个Firmicutes门上的数百个物种中，包括多种病原体和肠道菌群的共生成员，并且与被测菌株中的EET活性相关。这些发现表明，基于EET的生长能力更加普遍，并且在包括宿主相关的微生物群落和传染性疾病在内的各种环境中，对于电原细菌的建立了以前未被充分认识的相关性。

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  • 来源
    《Nature》 |2018年第7725期|140-144|共5页
  • 作者

    Light Samuel H.; Su Lin; Rivera-Lugo Rafael; Cornejo Jose A.; Louie Alexander; Iavarone Anthony T.; Ajo-Franklin Caroline M.; Portnoy Daniel A.;

  • 作者单位

    Univ Calif Berkeley, Dept Mol & Cell Biol, 229 Stanley Hall, Berkeley, CA 94720 USA;

    Lawrence Berkeley Natl Lab, Mol Foundry Mol Biophys & Integrated Bioimaging, Berkeley, CA USA;

    Univ Calif Berkeley, Dept Mol & Cell Biol, 229 Stanley Hall, Berkeley, CA 94720 USA;

    Lawrence Berkeley Natl Lab, Mol Foundry Mol Biophys & Integrated Bioimaging, Berkeley, CA USA;

    Univ Calif Berkeley, Dept Mol & Cell Biol, 229 Stanley Hall, Berkeley, CA 94720 USA;

    Univ Calif Berkeley, Chem Mass Spectrometry Facil QB3, Berkeley, CA 94720 USA;

    Lawrence Berkeley Natl Lab, Mol Foundry Mol Biophys & Integrated Bioimaging, Berkeley, CA USA;

    Univ Calif Berkeley, Dept Mol & Cell Biol, 229 Stanley Hall, Berkeley, CA 94720 USA;

  • 收录信息 美国《科学引文索引》(SCI);美国《工程索引》(EI);美国《生物学医学文摘》(MEDLINE);美国《化学文摘》(CA);
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